Size-based hydrodynamic rare tumor cell separation in curved microfluidic channels
Size-based hydrodynamic rare tumor cell separation in curved microfluidic channels
复制标题
弯曲微流体通道中基于尺寸的流体动力学稀有肿瘤细胞分离
DOI：
10.1063/1.4774311
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发表时间：
2013-01-01
期刊：
影响因子：
3.2
通讯作者：
Jiang, Xingyu
中科院分区：
文献类型：
--
作者：
Sun, Jiashu;Liu, Chao;Jiang, Xingyu
在这项工作中，我们提出了一个快速和连续的罕见的肿瘤细胞分离的基础上，在一个无标记的方式流体动力学效应。双螺旋微通道内惯性升力和迪恩阻力之间的竞争导致大肿瘤细胞和小血细胞的基于尺寸的细胞分离。通过数值模拟研究了弯曲微通道内流体动力分离的机理。用双螺旋通道对直径为5 μ m和15 μ m的聚苯乙烯颗粒的二元混合物进行的实验表明，在30 ml/h以上的流速下，分离纯度大于95%。通过双螺旋通道还实现了高通量（2.5 × 10（8）个细胞/min）和加标HeLa细胞和20 ×稀释血细胞的有效细胞分离（超过90%）。(C)2013年美国物理学会。[http：//dx.doi.org/10.1063/1.4774311]
In this work, we propose a rapid and continuous rare tumor cell separation based on hydrodynamic effects in a label-free manner. The competition between the inertial lift force and Dean drag force inside a double spiral microchannel results in the size-based cell separation of large tumor cells and small blood cells. The mechanism of hydrodynamic separation in curved microchannel was investigated by a numerical model. Experiments with binary mixture of 5- and 15-mu m-diameter polystyrene particles using the double spiral channel showed a separation purity of more than 95% at the flow rate above 30 ml/h. High throughput (2.5 x 10(8) cells/min) and efficient cell separation (more than 90%) of spiked HeLa cells and 20 x diluted blood cells was also achieved by the double spiral channel. (C) 2013 American Institute of Physics. [http://dx.doi.org/10.1063/1.4774311]