Chemotactic migration of bacteria in porous media
Chemotactic migration of bacteria in porous media
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DOI：
10.1016/j.bpj.2021.05.012
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发表时间：
2021-08-17
影响因子：
3.4
通讯作者：
Datta, Sujit S.
中科院分区：
文献类型：
--
作者：
Bhattacharjee, Tapomoy;Amchin, Daniel B.;Datta, Sujit S.
细菌的趋化性迁移--它们引导多细胞运动沿着化学梯度的能力--是农业、环境和医学过程的核心。然而，目前对迁移的理解是基于在散装液体中进行的研究，尽管许多细菌栖息在紧密的多孔介质中，如土壤，沉积物和生物凝胶。在这里，我们直接可视化了在没有任何其他施加的外力（例如，流动）。我们发现，孔隙尺度限制是一个强大的调节器的迁移。引人注目的是，细胞使用不同的主要机制来指导它们在限制中的运动，而不是在大量液体中。此外，限制显着改变的动态和形态的迁移人口的功能，可以描述的连续模型，但只有当标准的运动参数从他们的散装液体值大幅改变，以反映孔隙尺度限制的影响。因此，我们的工作提供了一个框架，以预测和控制细菌的迁移，一般活性物质，在复杂的环境中。
Chemotactic migration of bacteria-their ability to direct multicellular motion along chemical gradients-is central to processes in agriculture, the environment, and medicine. However, current understanding of migration is based on studies performed in bulk liquid, despite the fact that many bacteria inhabit tight porous media such as soils, sediments, and biological gels. Here, we directly visualize the chemotactic migration of Escherichia coli populations in well-defined 3D porous media in the absence of any other imposed external forcing (e.g., flow). We find that pore-scale confinement is a strong regulator of migration. Strikingly, cells use a different primary mechanism to direct their motion in confinement than in bulk liquid. Furthermore, confinement markedly alters the dynamics and morphology of the migrating population-features that can be described by a continuum model, but only when standard motility parameters are substantially altered from their bulk liquid values to reflect the influence of pore-scale confinement. Our work thus provides a framework to predict and control the migration of bacteria, and active matter in general, in complex environments.