Depletion of α-tocopherol and astaxanthin in Atlantic salmon (Salmo salar) affects autoxidative defense and fatty acid metabolism
Depletion of α-tocopherol and astaxanthin in Atlantic salmon (Salmo salar) affects autoxidative defense and fatty acid metabolism
复制标题
DOI：
10.1093/jn/130.7.1800
复制
发表时间：
2000-07-01
影响因子：
4.2
通讯作者：
Sargent, JR
中科院分区：
文献类型：
--
作者：
Bell, JG;McEvoy, J;Sargent, JR
两组大西洋鲑鱼在腌制后分别饲喂4种纯化饲料，分别添加维生素E和类胡萝卜素虾青素（Ax） （+E, +Ax），或添加维生素E或Ax （-E， +Ax和+E， -Ax）或缺乏维生素E和Ax (-E, -Ax) 22周。饲料对生长速度没有影响，但15%的饲料缺乏维生素E的鱼出现了广泛的脂质性肝脏退行性病变。组织中维生素E的浓度随饲料中维生素E含量的变化而变化，包括肝脏、肌肉、心脏、血浆、大脑和眼睛；与补充维生素E的饲料相比，维生素E缺乏饲料的鱼肝脏中的维生素E水平降低了3%，而眼睛中的维生素E水平仅降低了40%。研究发现，补充维生素E对组织维生素E浓度有交互作用，但仅在大脑中。饮食中维生素E和Ax的缺乏显著增加了分离肝细胞中[1-C-14] 18:3（n-3）和[1-C-14] 20:5（n-3）的去饱和和延长产物的恢复，这表明脂肪酸向长链高度不饱和产物的转化可以通过缺乏脂溶性抗氧化剂来刺激。维生素E和Ax在体外刺激微粒体脂质过氧化时能够减少丙二醛的形成，并降低血浆中8-异前列腺素的水平，从而支持了它们的抗氧化协同作用。这项研究的结果表明，大西洋鲑鱼中的维生素E和类胡萝卜素Ax都具有抗氧化功能。
Duplicate groups of Atlantic salmon post-smelts were fed four purified diets supplemented with both vitamin E and the carotenoid astaxanthin (Ax) (+E, +Ax), or supplemented with either vitamin E or Ax (-E, +Ax and +E, -Ax) or deficient in both vitamin E and Ax (-E, -Ax) for 22 wk. There were no effects of diet on growth rate, but an extensive lipoid liver degenerative lesion was observed in 15% of fish fed diets deficient in vitamin E. Tissue vitamin E concentrations varied in accordance with dietary vitamin E in liver, muscle, heart, plasma, brain and eye; levels were reduced to similar to 3% in liver but only to 40% in eye of fish fed diets deficient in vitamin E compared with those fed diets supplemented with vitamin E. An interactive sparing of Ax supplementation on tissue vitamin E concentration was observed, but only in brain. Dietary deficiency of both vitamin E and Ax significantly increased the recovery of desaturated and elongated products of both [1-C-14] 18:3(n-3) and [1-C-14] 20:5(n-3) in isolated hepatocytes, suggesting that conversion of fatty acids to their long-chain highly unsaturated products can be stimulated by a deficiency of lipid-soluble antioxidants. The antioxidant synergism of vitamin E and Ax was supported by their ability to reduce malondialdehyde formation in an in vitro stimulation of microsomal lipid peroxidation and to reduce plasma levels of 8-isoprostane. The results of this study suggest that both vitamin E and the carotenoid Ax have antioxidant functions in Atlantic salmon.