Effects of Monomers Eluted from Dental Resin Restoratives on Osteoblast-Like Cells
Effects of Monomers Eluted from Dental Resin Restoratives on Osteoblast-Like Cells
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DOI：
10.1002/jbm.b.31067
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发表时间：
2009-02-01
影响因子：
3.4
通讯作者：
Ebisu, Shigeyuki
中科院分区：
文献类型：
--
作者：
Imazato, Satoshi;Horikawa, Daisuke;Ebisu, Shigeyuki
树脂修饰的玻璃离聚体（RMGIC）已被证明对其表面的成骨细胞生长和分化具有抑制作用。本研究检验了树脂修复剂对成骨细胞不同程度的抑制作用取决于未聚合单体洗脱的假设。测定Bis-GMA/TEGDMA复合材料、MMA-树脂水泥或含HEMA的RMGIC固化标本中单体的释放量，并根据释放浓度分别在100-10 μ g/mL TEGDMA、10-1 μ g/mL MMA或400-50 μ g/mL HEMA中培养成骨样MC3T3-E1细胞。观察细胞增殖、碱性磷酸酶（ALP）活性、成骨细胞标志物表达和矿化组织形成。所测浓度的TEGDMA和MMA对MC3T3-E1的生长没有影响，对其分化和矿化也没有什么有害影响。相反，在200 μ g/mL以上时，HEMA抑制细胞增殖、ALP活性、骨钙素表达和矿化组织形成。这些结果表明，与洗脱试验中观察到的浓度相似的HEMA会影响成骨细胞的增殖、分化和矿化，这表明未反应的HEMA的洗脱可能是RMGIC对成骨细胞样细胞不利影响的主要成分，树脂修复剂对成骨细胞的影响可能取决于未聚合单体的释放特性。(c) 2008 Wiley期刊有限公司[J]中国生物医学工程学报，2009,31 (2):379 - 379
Resin-modified glass-ionomer (RMGIC) has been demonstrated to exhibit inhibition on the growth and differentiation of osteoblasts on its surface. In this study, the hypothesis that the different levels of inhibitory effects on osteoblasts of resin restoratives depend on elution of unpolymerized monomers was examined. Release of monomers from cured specimens of Bis-GMA/TEGDMA-composites, MMA-resin cements, or HEMA-containing RMGIC was determined and osteoblast-like MC3T3-E1 cells were cultured in the presence of 100-10 mu g/mL of TEGDMA, 10-1 mu g/mL of MMA, or 400-50 mu g/mL of HEMA according to the release concentrations. Cell proliferation, alkaline phosphatase (ALP) activity, the expression of osteoblastic markers, and mineralized tissue formation were evaluated. TEGDMA and MMA at the concentrations tested did not affect the growth of MC3T3-E1 and exhibited little harmful effects on their differentiation and mineralization. On the contrary, HEMA inhibited proliferation, ALP activities, the expression of osteocalcin, and mineralized tissue formation at 200 mu g/mL or more. These results indicate that HEMA at the concentrations similar to that observed in elution tests affected osteoblastic proliferation, differentiation, and mineralization, suggesting that elution of unreacted HEMA could be the main component of the adverse effects of RMGIC on osteoblast-like cells and influences of resin restoratives on the osteoblasts are possibly dependant on release characteristics of unpolymerized monomers. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 88B: 378-396, 2009