ELECTROPHORESIS BUFFERS FOR POLYACRYLAMIDE GELS AT VARIOUS PH
ELECTROPHORESIS BUFFERS FOR POLYACRYLAMIDE GELS AT VARIOUS PH
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DOI：
10.1016/0003-2697(82)90113-0
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发表时间：
1982-01-01
影响因子：
2.9
通讯作者：
MCLELLAN, T
中科院分区：
文献类型：
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作者：
MCLELLAN, T
连续电泳缓冲液被描述用于pH值范围为3.8至10.2的聚丙烯酰胺凝胶。缓冲液由酸性和碱性组分组成，pK值接近缓冲液的pH值。在长时间电泳期间，电极隔室中的pH保持在0.5 pH单位内。一些蛋白质在凝胶上用10种缓冲液中的每一种产生清晰的条带。缓冲液提供了凝胶电泳的pH范围的扩展，并且可能在蛋白质的遗传变异的检测和其他应用中是有用的。
Continuous electrophoresis buffers are described for polyacrylamide gels at pH values ranging from 3.8 to 10.2. The buffers consist of an acidic and a basic component with pK values near the pH of the buffer. The pH is maintained to within 0.5 pH unit in the electrode compartments during prolonged electrophoresis. Some proteins produce clear bands on gels with each of the 10 buffers. The buffers provide an expansion of the pH range of gel electrophoresis and are likely to be useful in the detection of genetic variation in proteins and in other applications.