FIELD PERFORMANCE OF ELITE TRANSGENIC MAIZE PLANTS EXPRESSING AN INSECTICIDAL PROTEIN DERIVED FROM BACILLUS-THURINGIENSIS
FIELD PERFORMANCE OF ELITE TRANSGENIC MAIZE PLANTS EXPRESSING AN INSECTICIDAL PROTEIN DERIVED FROM BACILLUS-THURINGIENSIS
复制标题
DOI：
10.1038/nbt0293-194
复制
发表时间：
1993-02-01
期刊：
影响因子：
--
通讯作者：
EVOLA, SV
中科院分区：
文献类型：
--
作者：
KOZIEL, MG;BELAND, GL;EVOLA, SV
我们使用微粒轰击将编码源自苏云金杆菌的CryIA（B）蛋白截短版本的合成基因引入玉米优良品系的未成熟胚中。使用CaMV 35S启动子或来自玉米的两种组织特异性启动子的组合表达该基因。使用两种启动子构型获得高水平的CryIA（B）蛋白。在田间条件下评价由转基因优良自交系植物与商业自交系杂交产生的杂交玉米植物对欧洲玉米螟的抗性。表达高水平杀虫蛋白的植物对该害虫的反复严重侵染表现出优异的抗性。
We introduced a synthetic gene encoding a truncated version of the CryIA(b) protein derived from Bacillus thuringiensis into immature embryos of an elite line of maize using microprojectile bombardment. This gene was expressed using either the CaMV 35S promoter or a combination of two tissue specific promoters derived from maize. High levels of CryIA(b) protein were obtained using both promoter configurations. Hybrid maize plants resulting from crosses of transgenic elite inbred plants with commercial inbred lines were evaluated for resistance to European corn borer under field conditions. Plants expressing high levels of the insecticidal protein exhibited excellent resistance to repeated heavy infestations of this pest.