Molecular characterization of ovine alpha-, beta(A)- and beta(B)-inhibin/activin alleles
Molecular characterization of ovine alpha-, beta(A)- and beta(B)-inhibin/activin alleles
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DOI：
10.1111/j.1439-0388.1996.tb00627.x
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发表时间：
1996-08-01
影响因子：
2.6
通讯作者：
Wassmuth, R
中科院分区：
文献类型：
--
作者：
Hiendleder, S;Leyhe, B;Wassmuth, R
绵羊抑制素/激活素基因被作为产羔数的候选QTL进行了研究。通过对5个不同繁殖力品种的RFLP研究，确定了4个INHA、2个INHBA和2个INHBB等位基因。Taqi INHBA等位基因的频率与所调查品种的平均产羔数相关(n=40-169)。对东弗里西亚奶羊(n=146)产羔记录的统计分析表明，INHBA基因对产羔数有显著的影响。对PCR产物的序列分析发现，INHBA基因编码区有几个核苷酸替换，其中一些会改变预测的氨基酸序列。三个抑制素/激活素基因各三个拷贝的基因组克隆和序列分析发现了额外的INHBA和INHBB等位基因，并揭示了编码区和调控元件的进一步序列多态。用原位杂交的方法定位了这三个基因。这排除了INHBB作为FEC(B)突变的位置。INHA和INHBA的归属通过连锁作图得到确认。
The ovine inhibin/activin genes were investigated as QTL candidates for litter size. An RFLP study with five breeds of differing fecundity identified 4 INHA, 2 INHBA and 2 INHBB alleles. The frequency of a TaqI INHBA allele was correlated with the average litter size of the investigated breeds (n=40-169). Statistical analysis of lambing records from East Friesian Milksheep (n=146) yielded significant INHBA gene effects on litter size. Sequence analysis of PCR products identified several nucleotide substitutions in coding regions of the INHBA gene, some of which change the predicted amino acid sequences. Genomic cloning and sequence analyses of three copies each of all three inhibin/activin genes identified additional INHBA and INHBB alleles, and revealed further sequence polymorphism in coding regions and regulatory elements. All three genes were mapped by in situ hybridization. This excluded INHBB as the site of the Fec(B) mutation. For INHA and INHBA, the assignments were confirmed by linkage mapping.