Effects of different harvesting seasons on antioxidant activity and phenolic content of prickly pear cladode juice.
Effects of different harvesting seasons on antioxidant activity and phenolic content of prickly pear cladode juice.
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DOI：
10.1016/j.jssas.2016.11.005
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发表时间：
2018-01-01
影响因子：
--
通讯作者：
le Meurlay, D.
中科院分区：
文献类型：
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作者：
Boutakiout, A.;Elothmani, D.;le Meurlay, D.
在春季、夏季和冬季三个采样期，研究了不同环境条件对两种不同叶状突起的酚类物质含量和抗氧化活性的影响。采用DPPH法、ABTS法和FRAP法对无刺仙人掌和多刺仙人掌的汁液中酚类物质含量和抗氧化活性进行了分析。采用高效液相色谱法测定了3月份采收的枝叶果汁中主要酚类物质的组成。总酚含量平均值为455.65~542.70 g GAEm L-1。DPPH法测得的抗氧化活性为1.78~4.10molTE mL-1，ABTS法测得的抗氧化活性为12.78~23.10moltemL-1，FRAP法测得的抗氧化活性为1.74~3.33moltemL-1。抗氧化剂活性和多酚类物质含量在夏季较高。与巨棘豆相比，无花果野生稻记录到的参数值显著高于巨棘豆。
The study was carried out to evaluate the effect of environmental conditions on the phenolic content and antioxidant activity of two distinct cladode genotypes under three sampling dates (spring, summer, and winter). The juice extracted from Opuntia ficus-indica (spineless) and Opuntia megacantha (spiny) genotypes was analyzed for their phenolics content and antioxidant activity using DPPH, ABTS, and FRAP methods. The composition of major phenolics substances of cladode juice harvested in March was determined by HPLC analysis. The average of the total phenolics content ranged from 455.65 to 542.70 g GAE mL-1. The antioxidant activity varied from 1.78 to 4.10 mol TE mL-1 by DPPH assay, 12.78-23.10 mol TE mL-1 by ABTS assay and 1.74-3.33 mol TE mL-1 by FRAP assay. Higher values of antioxidant activity and polyphenols content were registered in summer. O. ficus-indica recorded significantly higher values for the parameters compared to O. megacantha.