Clinical Evaluation of Multiplex Real-Time PCR Panels for Rapid Detection of Respiratory Viral Infections
Clinical Evaluation of Multiplex Real-Time PCR Panels for Rapid Detection of Respiratory Viral Infections
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DOI：
10.1002/jmv.22186
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发表时间：
2012-01-01
影响因子：
12.7
通讯作者：
Rinaldo, Charles R.
中科院分区：
文献类型：
--
作者：
Sanghavi, Sonali K.;Bullotta, Arlene;Rinaldo, Charles R.
呼吸道病毒感染是发病率和死亡率的主要原因之一，特别是在儿童、老年人和免疫功能受损的人中。快速识别病毒病原学对于排除非病毒感染、启动抗病毒治疗和限制感染传播至关重要。在同一试管中进行多个病毒基因靶点的多重检测具有在短时间内快速筛选大量潜在的病毒病原体的优点。本研究采用多重实时荧光定量聚合酶链式反应(RT-PCR)检测585例成人和儿童呼吸道病毒感染标本，包括有症状和无症状器官移植受者和非受者，用于呼吸道疾病的诊断和常规临床监测。多重PCR比多重免疫荧光培养法(R-MIX)更敏感，还可以检测到R-MIX小组没有涵盖的其他呼吸道病毒。在成人和儿童患者中，在有症状的患者中检测到的呼吸道病毒数量明显高于无症状的患者。疱疹病毒感染是器官移植受者下呼吸道感染的主要原因，而呼吸道合胞病毒是非器官移植患者尤其是儿童最常见的病原体。多重实时聚合酶链式反应检测呼吸道病毒具有快速鉴定病毒病原体的潜力。在这个新出现的病毒感染的时代，在多重聚合酶链式反应中增加新的病毒靶点将有助于确定患者管理和公共卫生流行病学。J.Med.维罗尔。2012年，84：162-169。(C)2011年威利期刊公司。
Respiratory viral infections are one of the leading causes of morbidity and mortality, particularly in children, the elderly and immunocompromised persons. Rapid identification of viral etiology is critical in ruling out non-viral infections, initiating antiviral treatment and limiting the spread of the infection. Multiplex assays of more than one viral gene target in a single tube have the advantage of rapid screening of a large number of potential viral pathogens in a short time. A multiplex real-time PCR assay was used in this study for detection of respiratory RNA and DNA viral infections in 728 specimens received from 585 adult and pediatric patients comprised of symptomatic and asymptomatic organ transplant recipients and non-recipients for diagnosis of respiratory illnesses and for routine clinical monitoring. Multiplex PCR was more sensitive than the multiplex immunofluoresence culture assay ( R-mix) and also detected additional respiratory viruses that were not covered by the R-mix panel. The number of respiratory viruses detected in symptomatic patients was significantly higher than asymptomatic patients in both adult and pediatric patients. Herpesviral infections were the predominant cause of lower respiratory tract infection in the organ transplant recipients, whereas respiratory syncytial virus was the most common pathogen in non-transplant patients particularly children. Multiplex real-time PCR for detection of respiratory viruses has the potential for rapid identification of viral pathogens. In this era of emerging viral infections, addition of newer viral targets to the multiplex PCR panels will be beneficial in determining both patient management and public health epidemiology. J. Med. Virol. 84:162-169, 2012. (C) 2011 Wiley Periodicals, Inc.