Effects of Xiaoyaosan on Stress-Induced Anxiety-Like Behavior in Rats: Involvement of CRF1 Receptor.
Effects of Xiaoyaosan on Stress-Induced Anxiety-Like Behavior in Rats: Involvement of CRF1 Receptor.
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逍遥散对大鼠应激性焦虑样行为的影响：CRF1受体的参与
DOI：
10.1155/2016/1238426
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Chen JX
中科院分区：
文献类型：
--
作者：
Jiang YM;Li XJ;Meng ZZ;Liu YY;Zhao HB;Li N;Yan ZY;Ma QY;Zhang HT;Chen JX
背景与逍遥散的抗抑郁作用相比，逍遥散抗焦虑作用的研究较少。Objective.观察逍遥散对慢性束缚应激（CIS）诱导的焦虑样行为的影响，并进一步探讨其作用是否与CRF 1 R信号通路有关。方法.将成年雄性SD大鼠随机分为五组（n = 12）：非应激对照组、媒介物处理（盐水，p.o.）组，逍遥散组（3.854g/kg，p.o.）对照组、溶剂治疗（手术）组和抗甲氨蝶呤治疗（手术）组。手术组双侧基底外侧杏仁核注入人工脑脊液（0.5 μL/侧）或CRF 1 R拮抗剂antalarmin（125 ng/0.5 μL，0.5 μL/侧）。除非应激对照组外，其余4组均于处理后30 min暴露于CIS（14 d，3 h/d）。第15天和第16天，所有动物均进行高架十字迷宫（EPM）和新奇抑制进食（NSF）测试。然后我们检测了杏仁核中CRF 1 R、pCREB和BDNF的表达。结果逍遥散或antalarmin慢性预处理可显著逆转应激大鼠升高的焦虑样行为和杏仁核中CRF 1 R和BDNF水平的上调。各组间pCREB无显著差异。结论.提示逍遥散在行为学测试中具有抗焦虑作用，其作用可能与杏仁核CRF 1 R信号通路有关。
Background. Compared with antidepressant activity of Xiaoyaosan, the role of Xiaoyaosan in anxiety has been poorly studied. Objective. To observe the effects of Xiaoyaosan on anxiety-like behavior induced by chronic immobilization stress (CIS) and further explore whether these effects were related to CRF1R signaling. Methods. Adult male SD rats were randomly assigned to five groups (n = 12): the nonstressed control group, vehicle-treated (saline, p.o.) group, Xiaoyaosan-treated (3.854 g/kg, p.o.) group, vehicle-treated (surgery) group, and antalarmin-treated (surgery) group. Artificial cerebrospinal fluid (0.5 μL/side) or CRF1R antagonist antalarmin (125 ng/0.5 μL, 0.5 μL/side) was bilaterally administered into the basolateral amygdala in the surgery groups. Except for the nonstressed control group, the other four groups were exposed to CIS (14 days, 3 h/day) 30 minutes after treatment. On days 15 and 16, all animals were subjected to the elevated plus-maze (EPM) and novelty suppressed feeding (NSF) test. We then examined the expression of CRF1R, pCREB, and BDNF in the amygdala. Results. Chronic pretreatment with Xiaoyaosan or antalarmin significantly reversed elevated anxiety-like behavior and the upregulated level of CRF1R and BDNF in the amygdala of stressed rats. pCREB did not differ significantly among the groups. Conclusions. These results suggest that Xiaoyaosan exerts anxiolytic-like effects in behavioral tests and the effects may be related to CRF1R signaling in the amygdala.