Adenovirus-mediated Ink4a/ARF gene transfer significantly suppressed the growth of pancreatic carcinoma cells
Adenovirus-mediated Ink4a/ARF gene transfer significantly suppressed the growth of pancreatic carcinoma cells
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DOI：
10.4161/cbt.4.12.2183
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发表时间：
2005-10
影响因子：
3.6
通讯作者：
Fang-Yuan Chen;Yiting Li;Zhaohui Lu;Jie Gao;Jie Chen
中科院分区：
文献类型：
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作者：
Fang-Yuan Chen;Yiting Li;Zhaohui Lu;Jie Gao;Jie Chen
目的：探讨腺病毒介导的p14 ARF和p16 INK 4a转移对人胰腺癌细胞系生长和凋亡的影响。结果：胰腺癌细胞系PC-7、PANC-1和MIA PaCa-2（p14 ARF和p16 INK 4a-/-）。表达p14 ARF基因的重组腺病毒载体（Adp 14）感染PC-7（p53 wt）和MIA PaCa-2（p53 mt）细胞，与对照载体相比，在体外和体内均表现出明显的细胞生长抑制作用，而PANC-1细胞（p53 mt）则没有这种作用。腺病毒载体p16 INK 4a基因（Adp 16）感染上述3株细胞后，在体内外均表现出生长迟缓和衰老的表型。结论：腺病毒介导的p14 ARF和p16 INK 4a基因转染对胰腺癌细胞的生长抑制作用Adp 14的作用部分取决于p53基因在这些细胞中的状态。
Objectives: To determine the effects of adenovirus-mediated transfer of p14ARF and p16INK4a on growth and apoptosis of human pancreatic carcinoma cell lines. Results: Pancreatic carcinoma cell lines, PC-7, PANC-1 and MIA PaCa-2 (p14ARF and p16INK4a -/-), were used. PC-7 (p53 wt) and MIA PaCa-2 (p53 mt) cells infected with recombinant adenovirus vector expressing p14ARF gene (Adp14) showed significant inhibition of cell growth compared with control vector in vitro and in vivo, whereas PANC-1 cells (p53 mt) did not show such effects. Those three cell lines exhibited growth retardation and senescence phenotype after infected with the adenovirus vector containing p16INK4a gene (Adp16) in vivo and in vitro. Conclusions: Adenovirus-mediated transfer of p14ARF and p16INK4a produces significant growth suppression of pancreatic carcinoma cells in vitro and in vivo. The effects of Adp14 partly depend on the status of p53 gene in those cells.