DEVELOPMENTAL REGULATION OF BETA-GLOBIN GENE SWITCHING
DEVELOPMENTAL REGULATION OF BETA-GLOBIN GENE SWITCHING
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DOI：
10.1016/0092-8674(88)90005-0
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发表时间：
1988-10-07
期刊：
影响因子：
64.5
通讯作者：
ENGEL, JD
中科院分区：
文献类型：
--
作者：
CHOI, ORB;ENGEL, JD
鸡红细胞特异性增强子位于成体E-珠蛋白基因和胚胎E-珠蛋白基因之间的基因间隔区。在这篇文章中，我们证明了P-珠蛋白增强子刺激这两个基因的转录。然而，E-珠蛋白受到不适当的调控，因为它在胚胎和成人红细胞中都有表达。当两个基因都存在于一个质粒上时，观察到对它们进行适当的阶段特异性调控。通过对缺失和替换突变体的分析，我们得出结论，P-珠蛋白组织和发育阶段特异性调控是由P-珠蛋白增强子与成人B-珠蛋白启动子中的正调控元件-发育阶段选择元件(SSE)相互作用所介导的。
A chicken erythroid cell-specific enhancer is located in the intergenic region between the adult fi-and embryonic E-globin genes. In this paper we show that the P-globin enhancer stimulates transcription of both genes. E-Globin is, however, inappropriately regulated since it is expressed in both embryonic and adult red blood cells. Appropriate stage-specific regulation is observed for both genes when they are present on one plasmid. By analysis of deletion and substitution mutants, we conclude that P-globin tissue-and developmental stage-specific regulation is mediated by interaction of the P-globin enhancer with a positive regulatory element within the adult B-globin promoter, the developmental stage selector element (SSE).