CORRELATION BETWEEN THE ABUNDANCE OF YEAST TRANSFER-RNAS AND THE OCCURRENCE OF THE RESPECTIVE CODONS IN PROTEIN GENES - DIFFERENCES IN SYNONYMOUS CODON CHOICE PATTERNS OF YEAST AND ESCHERICHIA-COLI WITH REFERENCE TO THE ABUNDANCE OF ISOACCEPTING TRANSFER-RNAS
CORRELATION BETWEEN THE ABUNDANCE OF YEAST TRANSFER-RNAS AND THE OCCURRENCE OF THE RESPECTIVE CODONS IN PROTEIN GENES - DIFFERENCES IN SYNONYMOUS CODON CHOICE PATTERNS OF YEAST AND ESCHERICHIA-COLI WITH REFERENCE TO THE ABUNDANCE OF ISOACCEPTING TRANSFER-RNAS
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DOI：
10.1016/0022-2836(82)90250-9
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发表时间：
1982-01-01
影响因子：
5.6
通讯作者：
IKEMURA, T
中科院分区：
文献类型：
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作者：
IKEMURA, T
目前已测序的酵母核基因的同义密码子选择模式存在相似性，但这些基因编码不同类型的蛋白质分子，其模式与E. coli基因。基于E.大肠杆菌转移RNA及其密码子识别的性质与反密码子摆动位置上的修饰核苷酸有关，大肠杆菌常见的同义密码子选择的特征模式。大肠杆菌的基因几乎已经被完全解释了。酵母S.通过双向聚丙烯酰胺凝胶电泳分离酿酒酵母，并根据细胞中的分子数量测量纯化的tRNA分子的相对丰度。对于酵母的每个核基因，发现tRNA丰度和密码子选择之间的强相关性，但是对于2 μ g/ml，该相关性不太显著。质粒基因根据E.大肠杆菌基因中，酵母核基因中密码子偏好的顺序是基于酵母同功tRNA的丰度和在其反密码子处修饰的核苷酸的性质来预测的。同义密码子之间的预测和实际偏好之间的明确相关性被揭示，表明酵母基因中的密码子选择也受到tRNA可用性和其密码子识别性质的组合的限制。那么这两种生物体之间同义密码子使用的差异可以归因于这两个因素的差异。
There exists a similarity among the synonymous codon choice patterns of the yeast nuclear genes that have been sequenced thus far, although these genes encode different types of protein molecules and the patterns are significantly different from those of E. coli genes. Based on constraints caused by the availability of E. coli transfer RNA and the nature of their codon recognition related to the modified nucleotides at the anticodon wobble position, the characteristic patterns of synonymous codon choice commonly found for E. coli genes have been almost completely explained. tRNA of the yeast S. cerevisiae were separated by 2-dimensional polyacrylamide gel electrophoresis, and the relative abundance of purified tRNA molecules was measured on the basis of molecular numbers in cells. A strong correlation between tRNA abundance and codon choice was found for each nuclear gene of yeast, but the correlation was less significant for 2.mu. plasmid genes. According to the criteria proposed for E. coli genes the order of codon preference in yeast nuclear genes was predicted based on the abundance of yeast isoaccepting tRNA, and on the nature of the modified nucleotides at their anticodons. Clear correlations between predictions and the actual preferences among synonymous codons were revealed, indicating that the codon choices in yeast genes are also constrained by a combination of tRNA availability and nature of its codon recognition. Then the difference in synonymous codon use between the 2 organisms can be attributed to the difference in these 2 factors.