Microscopic and Ultrastructural Changes of the Intestine in Abdominal Compartment Syndrome
Microscopic and Ultrastructural Changes of the Intestine in Abdominal Compartment Syndrome
复制标题
DOI：
10.1080/08941930903214719
复制
发表时间：
2009-01-01
影响因子：
1.9
通讯作者：
Li, Jieshou
中科院分区：
文献类型：
--
作者：
Gong, Guanwen;Wang, Pengfei;Li, Jieshou
背景资料：本研究旨在观察腹腔内高压（IAH）20 mmHg时小肠绒毛的显微改变和紧密连接的超微结构变化。材料和方法：研究40只Sprague-Daley大鼠（250 +/- 25 g），分为4组（n = 10）。在30只动物中，分别将腹腔内压升高至20 mmHg持续1 h、2 h和4 h。其他人作为对照。将肠标本进行苏木精和伊红染色进行组织病理学检查，并使用扫描电子显微镜进行超微结构分析。结果如下：组织病理学检查显示不同程度的变化，从绒毛上皮细胞肿胀和变性到绒毛广泛剥脱和塌陷。超微结构分析显示，扩张的紧密连接和细胞旁空间的存在下IAH。损伤程度与IAH时间有关。结论：IAH增加导致肠上皮细胞明显损伤，肠紧密连接明显扩张，导致粘膜屏障通透性增加。这可能解释了为什么IAH经常与细菌移位和脓毒症有关。
Background: The purpose of the study was to examine themicroscopic changes of intestine villus and ultrastructural changes of tight junction in the presence of intra-abdominal hypertension (IAH) of 20 mmHg. Materials and Methods: Forty Sprague-Daley rats (250 +/- 25 g) divided into four groups (n = 10) were studied. In 30 animals, the intra-abdominal pressure was increased to 20 mmHg for 1 hr, 2 hr, and 4 hr, respectively. The others served as controls. The specimens of the intestines were submitted to histopathology using hematoxylin and eosin staining, and to ultrastructural analysis using a scanning electron microscope. Results: The histopathological study revealed different degrees of changes ranging from swelling and degeneration of villous epithelial cells to extensive denudation and collapse of the villus. Ultrastructural analysis revealed dilatation of the tight junctions and paracellular spaces in the presence of IAH. The severity of the damage was related to the time of IAH. Conclusions: Increased IAH caused a significant damage to the intestinal epithelium and a marked dilatation of intestinal tight junction, leading to the increased mucosal barrier permeability. It may explain why IAH was often associated with bacterial translocation and sepsis.