Expression of RANTES by bronchoalveolar lavage cells in nonsmoking patients with interstitial lung diseases
Expression of RANTES by bronchoalveolar lavage cells in nonsmoking patients with interstitial lung diseases
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DOI：
10.1165/ajrcmb.18.4.2868
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发表时间：
1998-04-01
影响因子：
6.4
通讯作者：
Kawakami, Y
中科院分区：
文献类型：
--
作者：
Kodama, N;Yamaguchi, E;Kawakami, Y
最近的重点放在趋化细胞因子的作用，称为趋化因子，以解释炎症细胞在肺中的积累，可能先于或伴随肺间质性肺疾病的肺纤维化。我们假设，RANTES，C-C趋化因子的成员，是这样的趋化因子之一。对20例结节病患者、10例胶原血管病相关性间质性肺炎（CVD-IP）患者、10例特发性肺纤维化（IPF）患者和8例健康志愿者（HV）进行支气管肺泡灌洗，所有患者均为从不吸烟者。我们通过竞争性逆转录-聚合酶链反应（RT-PCR）技术半定量自发RANTES mRNA表达，并通过酶联免疫吸附试验测定RANTES蛋白水平。各疾病组支气管肺泡灌洗液（BALF）细胞RANTES mRNA表达及BALF中RANTES蛋白水平均显著高于HV组。结节病和CVD-IP患者BALF细胞RANTES mRNA表达与BALF淋巴细胞增多呈显著正相关。外周血单个核细胞表达的RANTES mRNA的量和血清中RANTES蛋白的水平在所有研究组之间没有差异。我们的研究表明，半定量RT-PCR方法测定细胞因子mRNA的表达在体内的适应性。我们的研究结果表明，RANTES可能是一种趋化因子，参与了一些不同的间质性肺疾病的肺中的炎症细胞的积累的机制。
Emphasis has recently been placed on the roles of chemotactic cytokines called chemokines to explain the accumulation of inflammatory cells in the lung that may precede or accompany pulmonary fibrosis in interstitial lung diseases. We hypothesized that RANTES, a member of the C-C chemokines, is one such chemokine. Bronchoalveolar lavage was done in 20 patients with sarcoidosis, 10 patients with interstitial pneumonia associated with collagen vascular disease (CVD-IP), 10 patients with idiopathic pulmonary fibrosis (IPF), and eight healthy volunteers (HV), all of whom were never-smokers. We semiquantitated the spontaneous RANTES mRNA expression by a competitive reverse transcription-polymerase chain reaction (RT-PCR) technique, and measured the levels of RANTES protein by enzyme-linked immunosorbent assay. In all disease groups the expression of RANTES mRNA by bronchoalveolar lavage fluid (BALF) cells and the levels of RANTES protein in BALF were significantly increased compared with those in HV. Patients with sarcoidosis and CVD-IP had a significant positive correlation between the expression of RANTES mRNA by BALF cells and BALF lymphocytosis. The amounts of RANTES mRNA expressed by peripheral blood mononuclear cells and the levels of RANTES protein in serum did not differ among all study groups. Our study demonstrates the adaptability of a semiquantitative RT-PCR method for determining cytokine mRNA expression in vivo. Our results suggest that RANTES may be one of the chemokines that are involved in the mechanism for the accumulation of inflammatory cells in the lung of some distinct interstitial lung diseases.