In vitro human skin permeation and decontamination of 2-chloroethyl ethyl sulfide (CEES) using Dermal Decontamination Gel (DDGel) and Reactive Skin Decontamination Lotion (RSDL)
In vitro human skin permeation and decontamination of 2-chloroethyl ethyl sulfide (CEES) using Dermal Decontamination Gel (DDGel) and Reactive Skin Decontamination Lotion (RSDL)
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DOI：
10.1016/j.toxlet.2018.04.015
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发表时间：
2018-07-01
影响因子：
3.5
通讯作者：
Maibach, Howard
中科院分区：
文献类型：
--
作者：
Cao, Yachao;Hui, Xiaoying;Maibach, Howard
本研究比较了DDGel和RSDL对人体皮肤的体外去污效果。使用体外人皮肤模型进行实验，其中皮肤安装在流通扩散池上。通过用液体闪烁光谱仪测量放射性来定量去污后从皮肤表面去除的（14）-C CEES的质量。两种去污剂都能从皮肤上去除82%以上的CEES。与RSDL相比，DDGel皮肤去污显著降低了毒物量。DDGel和RSDL在角质层（SC）、活表皮、真皮和全身吸收中的平均CEES分别为0.12和0.55%（P < 0.01）、0.31和0.95%（p < 0.01）、1.08和2.92%（p < 0.05）、3.13和6.34%（p < 0.05）。DDGel显示出比RSDL更高的去污效率（两倍去污功效因子，DEF），并有效地从皮肤表面去除化学品，重要的是从SC反萃取，并显着减少化学品渗透到皮肤和全身吸收。因此，DDGel可以作为下一代皮肤去污平台技术用于军事和民用应用。
This study compared the efficiency for in vitro human skin decontamination using DDGel and RSDL. Experiments were performed using in vitro human skin models, in which skin was mounted onto Flow-Through diffusion cells. The mass of (14)-C CEES removed from skin surface after decontamination was quantitated by measuring radioactivity with a liquid scintillation spectrometer. Both decontaminants removed more than 82% of CEES from skin. DDGel skin decontamination significantly reduced toxicant amount when compared to RSDL. Mean CEES remaining in stratum corneum (SC), viable epidermis, dermis, and systemic absorption of DDGel and RSDL were, 0.12 and 0.55% (P < 0.01), 0.31 and 0.95% (p < 0.01), 1.08 and 2.92% (p < 0.05), 3.13 and 6.34% (p < 0.05), respectively. DDGel showed higher decontamination efficiency (twice decontamination efficacy factor, DEF) than RSDL and efficiently removed chemicals from the skin surface, importantly back-extracted from the SC, and significantly reduced both chemical penetration into skin and systemic absorption. Thus, DDGel can offer a potential as a next generation skin decontamination platform technology for military and civilian applications.