Taxonomic Characterization and Experimental Host Ranges of Four Newly Recorded Species of Alternaria from Japan
Taxonomic Characterization and Experimental Host Ranges of Four Newly Recorded Species of Alternaria from Japan
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DOI：
10.1111/jph.12108
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发表时间：
2013-09
影响因子：
1.5
通讯作者：
J. Nishikawa;C. Nakashima
中科院分区：
文献类型：
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作者：
J. Nishikawa;C. Nakashima
链格孢属真菌是重要的植物病原菌。在这里，我们确定了三种新的日本真菌区系：链格孢celosiae，链格孢crassa和链格孢petroselini。我们提出了一个新的名称为A。celosiae（E.G. Simmons & Holcomb）Lawrence，Park & Pryor，A. celosiae（Tassi）O.沙乌地为了表征这些和第四形态分类群，Alternaria alstroemeriae，这是最近加入到日本的真菌区系，一个综合的物种概念进行了测试。我们确定了每个分离物的宿主范围使用接种试验和分析其系统发育位置使用的内部转录间隔区rDNA序列。我们的A. Alstroemeriae分离物严格限于Alstroemeria sp.（Alstroemeriaceae），但该种与其他小孢子链格孢属的种在遗传学上无法区分。鸡冠花链格孢菌plumosa（苋科）对三色苋（Amaranthus tricolor）、串爪莲子草（Alternanthera paronychioides）也有致病性，对Gomphrena globosa（苋科）也有较弱的致病性，并与原Nimbya celosiae形成一个分支。曼陀罗（Datura stramonium）上的厚链格孢菌（Alternaria crassa）对茄科植物的Brugmansia × candida和辣椒（Capsicum annuum）也具有致病性，但对其他同属植物不具有致病性;在遗传学上，它属于具有丝状喙的大孢子种的一个分支。形态相似性、系统发育关系和实验寄主范围表明，A. crassa、Alternaria capsici和Alternaria daturicola为同一种。岩芹链格孢（Alternaria petroselini）是伞形科（Apiaceae）皱叶岩芹（Petroselinum crispum）上的一种重要病原菌，对伞形科（Apiaceae）的柴胡、芫荽、Seliaeae和Scandiceae等代表植物以及伞形科（Apiaceae）的5种植物均有致病性。形态学和遗传学均表明A. petroselini和Alternaria selini。
Alternaria fungi are important plant pathogens. Here, we identified three species new to the Japanese mycoflora: Alternaria celosiae, Alternaria crassa and Alternaria petroselini. We proposed a new name for A. celosiae (E.G. Simmons & Holcomb) Lawrence, Park & Pryor, a later homonym of A. celosiae (Tassi) O. Săvul. To characterize these and a fourth morphological taxon, Alternaria alstroemeriae, which was recently added to Japan's mycoflora, an integrated species concept was tested. We determined the host range of each isolate using inoculation tests and analysed its phylogenetic position using sequences of the internal transcribed spacer rDNA. The pathogenicity of our A. alstroemeriae isolate was strictly limited to Alstroemeria sp. (Alstroemeriaceae), but the species was phylogenetically indistinguishable from other small-spored Alternaria. Alternaria celosiae on Celosia argentea var. plumosa (Amaranthaceae) was also pathogenic to Amaranthus tricolor, to Alternanthera paronychioides and weakly to Gomphrena globosa (all Amaranthaceae) and formed a clade with the former Nimbya celosiae. Alternaria crassa on Datura stramonium (Solanaceae) was also pathogenic to Brugmansia × candida and Capsicum annuum in Solanaceae, but not to other confamilial plants; phylogenetically it belonged to a clade of large-spored species with filamentous beaks. Morphological similarity, phylogenetic relationship and experimental host range suggested that A. crassa, Alternaria capsici and Alternaria daturicola were conspecific. Alternaria petroselini on Petroselinum crispum (Apiaceae) was pathogenic to five species in the tribe Apieae as well as representatives of Bupleureae, Coriandreae, Seliaeae and Scandiceae in Apiaceae. Both phylogeny and morphology suggested conspecificity between A. petroselini and Alternaria selini.