Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response.
Coordinated functions of WSS1, PSY2 and TOF1 in the DNA damage response.
复制标题
WSS1、PSY2 和 TOF1 在 DNA 损伤反应中的协调功能。
DOI：
10.1093/nar/gkh994
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Romesberg,FloydE
中科院分区：
文献类型：
--
作者：
O'Neill,BryanM;Hanway,Denise;Winzeler,ElizabethA;Romesberg,FloydE
The stabilization and processing of stalled replication forks is required to maintain genome integrity in all organisms. In an effort to identify novel proteins that might be involved in stabilizing stalled replication forks,Saccharomyces cerevisiaemutantwss1Δ was isolated from a high-throughput screening of ∼5000 deletion strains for genes involved in the response to continuous, low-intensity UV irradiation. Disruption ofWSS1resulted in synergistic increases in UV sensitivity with null mutants of genes involved in recombination (RAD52) and cell cycle control (RAD9andRAD24). WSS1 was also found to interact genetically withSGS1,TOP3,SRS2andCTF4, which are involved in recombination, repair of replication forks and the establishment of sister chromatid cohesion. A yeast two-hybrid screen identified a potential physical interaction between Wss1 and both Psy2 and Tof1. Genetic interactions were also detected betweenPSY2andTOF1, as well as between each gene andRAD52andSRS2, and betweenWSS1andTOF1. Tof1 is known to be involved in stabilizing stalled replication forks and our data suggest that Wss1 and Psy2 similarly function to stabilize or process stalled or collapsed replication forks.
