TRPV1-lineage neurons are required for thermal sensation
TRPV1-lineage neurons are required for thermal sensation
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DOI：
10.1038/emboj.2010.325
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发表时间：
2011-02-02
期刊：
影响因子：
11.4
通讯作者：
Hoon, Mark A.
中科院分区：
文献类型：
--
作者：
Mishra, Santosh K.;Tisel, Sarah M.;Hoon, Mark A.
离子通道TRPV 1被认为是有害热的主要传感器，但令人惊讶的是，缺乏TRPV 1的动物仍然对升高的温度表现出显着的反应。在这项研究中，我们探索了TRPV 1表达神经元在躯体感觉中的作用，通过产生小鼠，其中该谱系的细胞被选择性标记或消融。我们的数据表明，TRPV 1是许多伤害感受器的胚胎标记，包括所有TRPV 1和TRPM 8神经元以及许多Mrg表达神经元。缺乏这些细胞的突变小鼠对热或冷完全不敏感，但与此形成鲜明对比的是，它们保留了正常的触觉和机械性疼痛感。这些动物也表现出有缺陷的体温控制和失去瘙痒和疼痛的反应，以有效的化学介质。与以前的细胞消融研究一起，我们的研究结果定义和划定了TRPV 1-和TRPM 8-神经元在温度感觉，温度调节和伤害感受中的作用，从而显着扩展了标记线在体感编码中的概念。The EMBO Journal（2011）30，582-593. doi：10.1038/daj.2010.325; 2010年12月7日在线发布
The ion-channel TRPV1 is believed to be a major sensor of noxious heat, but surprisingly animals lacking TRPV1 still display marked responses to elevated temperature. In this study, we explored the role of TRPV1-expressing neurons in somatosensation by generating mice wherein this lineage of cells was selectively labelled or ablated. Our data show that TRPV1 is an embryonic marker of many nociceptors including all TRPV1- and TRPM8-neurons as well as many Mrg-expressing neurons. Mutant mice lacking these cells are completely insensitive to hot or cold but in marked contrast retain normal touch and mechanical pain sensation. These animals also exhibit defective body temperature control and lose both itch and pain reactions to potent chemical mediators. Together with previous cell ablation studies, our results define and delimit the roles of TRPV1- and TRPM8-neurons in thermosensation, thermoregulation and nociception, thus significantly extending the concept of labelled lines in somatosensory coding. The EMBO Journal (2011) 30, 582-593. doi:10.1038/emboj.2010.325; Published online 7 December 2010