Expression and regulation of the early auxin-responsive Aux/IAA genes during strawberry fruit development
Expression and regulation of the early auxin-responsive Aux/IAA genes during strawberry fruit development
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DOI：
10.1007/s11033-010-0216-x
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发表时间：
2011-02-01
影响因子：
2.8
通讯作者：
Lu, Wang-jin
中科院分区：
文献类型：
--
作者：
Liu, Du-juan;Chen, Jian-ye;Lu, Wang-jin
植物激素生长素转录激活 Aux/IAA 基因。生长素在调节草莓果实生长和成熟中发挥着重要作用，Aux/IAA基因已在拟南芥、水稻和番茄中得到广泛研究，但有关草莓果实的信息很少。在目前的工作中，从草莓果实中分离并鉴定了两个全长的早期生长素响应 Aux/IAA 基因，分别称为 FaAux/IAA1 和 FaAux/IAA2。此外，还研究了两个FaAux/IAA基因在果实发育过程中的表达谱，以及萘乙酸（NAA）对其在两个不同发育阶段的果实表达的影响。结果表明，FaAux/IAA1和FaAux/IAA2转录本在果实发育早期水平很高，而在成熟期（白色期后）急剧下降。此外，在大绿果和白果期施用NAA明显增加了FaAux/IAA1和FaAux/IAA2转录本的积累。这些数据表明，FaAux/IAA1 和 FaAux/IAA2 基因的表达可能参与早期果实发育，FaAux/IAA 转录本的增强可能至少或部分归因于生长素诱导的果实生长和草莓果实成熟的延迟。
The plant hormone auxin transcriptionally activates Aux/IAA genes. Auxin plays an important role in regulating fruit growth and ripening of strawberry and Aux/IAA genes have been extensively studied in Arabidopsis, rice and tomato, but little information is available on strawberry fruit. In the present work, two full-length of early auxin-responsive Aux/IAA genes, termed FaAux/IAA1 and FaAux/IAA2 respectively, were isolated and characterized from strawberry fruit. Moreover, the expression profiles of two FaAux/IAA genes during fruit development, and the effect of naphthalene acetic acid (NAA) on their expressions of fruits at two different developmental stages were also investigated. The results showed that the levels of FaAux/IAA1 and FaAux/IAA2 transcripts were very high at early stage of fruit development, and decreased sharply at ripening stage (after white stage). In addition, NAA applied at the stage of large green and white fruit obviously increased the accumulations of FaAux/IAA1 and FaAux/IAA2 transcripts. These data suggested that the expressions of both FaAux/IAA1 and FaAux/IAA2 genes were likely to be involved in early fruit development, and the enhancement of FaAux/IAAs transcripts might be attributed at least or partially to auxin-induced fruit growth and delayed fruit ripening of strawberry.