Metabolic profiling of an alcoholic fatty liver in zebrafish (Danio rerio)
Metabolic profiling of an alcoholic fatty liver in zebrafish (Danio rerio)
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DOI：
10.1039/c2mb25073j
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发表时间：
2012-01-01
影响因子：
--
通讯作者：
Hwang, Geum-Sook
中科院分区：
文献类型：
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作者：
Jang, Zi-Hey;Chung, Hee-Chung;Hwang, Geum-Sook
斑马鱼（Danio rerio）正在成为一种流行的发育生物学模型，用于研究疾病和药物发现。在这项研究中，我们使用斑马鱼疾病模型对酒精性脂肪肝进行了基于质子核磁共振谱（h -1 nmr）和气相色谱-质谱（GC/MS）的代谢分析。我们检查了斑马鱼对照组和酒精性脂肪肝组之间的代谢差异，以确定酒精性脂肪肝的代谢是如何被调节的。多因素统计分析显示，对照组与酒精性脂肪肝组之间存在显著性差异。酒精性脂肪肝组异亮氨酸、乙酸盐、琥珀酸盐、胆碱、肌酸、乙酰乙酸盐、3-羟基丁酸盐（3HB）、葡萄糖醛酸乙酯（EtG）、乳酸/丙酮酸比、脂肪酸和胆固醇的排泄增加，柠檬酸盐、天冬氨酸、酪氨酸、甘氨酸、葡萄糖、丙氨酸、甜菜碱和麦芽糖的排泄减少。在脂肪肝组中发现的代谢物与长期饮酒有关，这会导致氧化还原（氧化还原）变化和氧化应激。这项研究表明，斑马鱼模型的整体代谢物分析可以为酒精性脂肪肝的代谢变化提供见解。
Zebrafish (Danio rerio) is becoming a popular developmental biology model to study diseases and for drug discovery. In this study, we performed proton nuclear magnetic resonance spectroscopy (H-1-NMR)- and gas chromatography-mass spectrometry (GC/MS)-based metabolic profiling of an alcoholic fatty liver using a zebrafish disease model. We examined metabolic differences between the control and alcoholic fatty liver groups in zebrafish to determine how metabolism in an alcoholic fatty liver is regulated. Multivariate statistical analysis showed a significant difference between the control and alcoholic fatty liver groups. The alcoholic fatty liver group showed increased excretion of isoleucine, acetate, succinate, choline, creatine, acetoacetate, 3-hydroxybutyrate (3HB), ethyl glucuronide (EtG), lactate/pyruvate ratio, fatty acids, and cholesterol, and decreased excretion of citrate, aspartate, tyrosine, glycine, glucose, alanine, betaine, and maltose. Metabolites identified in the fatty liver groups were associated with long-term alcohol consumption, which causes both oxidation-reduction (redox) changes and oxidative stress. This study suggests that global metabolite profiling in a zebrafish model can provide insights into the metabolic changes in an alcoholic fatty liver.