Serine Palmitoyltransferase, the First Step Enzyme in Sphingolipid Biosynthesis, Is Involved in Nonhost Resistance
Serine Palmitoyltransferase, the First Step Enzyme in Sphingolipid Biosynthesis, Is Involved in Nonhost Resistance
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DOI：
10.1094/mpmi-22-1-0031
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发表时间：
2009-01-01
影响因子：
3.5
通讯作者：
Terauchi, Ryohei
中科院分区：
文献类型：
--
作者：
Takahashi, Yoshihiro;Berberich, Thomas;Terauchi, Ryohei
本氏烟草（Nicotiana benthamiana）cDNAs的过表达筛选鉴定了丝氨酸棕榈酰转移酶（SPT）的LCB 2亚基的基因作为过敏反应样细胞死亡的有效诱导物。NbLCB 2的吡哆醛5 '-磷酸结合位点是其作为细胞死亡诱导剂的功能所必需的。NbLCB 2 mRNA在非宿主病原体菊苣假单胞菌感染后积累。抗性N.在NbLCB 2-和NbLCB 1-沉默的植物中，通过用SPT抑制剂处理，本塞姆氏菌对菊苣疫霉的抗性受到损害。这些结果表明，鞘脂的生物合成是N. benthamiana对抗P. cichorii。
An overexpression screen of Nicotiana benthamiana cDNAs identified a gene for the LCB2 subunit of serine palmitoyltransferase (SPT) as a potent inducer of hypersensitive response-like cell death. The pyridoxal 5'-phosphate binding site of NbLCB2 is required for its function as a cell death inducer. NbLCB2 mRNA is accumulated after infection by nonhost pathogen Pseudomonas cichorii. Resistance of N. benthamiana against P. cichorii was compromised by treatment with an SPT inhibitor and in NbLCB2- and NbLCB1-silenced plants. These results suggest that biosynthesis of sphingolipids is necessary for the nonhost resistance of N. benthamiana against P. cichorii.