Structural changes in the inner ear over time studied in the experimentally deafened guinea pig
Structural changes in the inner ear over time studied in the experimentally deafened guinea pig
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DOI：
10.1002/jnr.23824
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发表时间：
2017-03-01
影响因子：
4.2
通讯作者：
Ulfendahl, Mats
中科院分区：
文献类型：
--
作者：
Fransson, Anette;Ulfendahl, Mats
今天，人工耳蜗(CI)可以显著恢复听觉功能，即使是对听力严重受损的人也是如此。由于CI的疗效主要依赖于残留的螺旋神经节神经元(SGN)，因此了解耳聋后听神经元退化过程的时间线是很重要的。用新霉素经鼓室致聋豚鼠，记录听性脑干反应(ABR)，在不同时间点处死豚鼠。观察SGN的丢失以及胞体和核体积的变化。为了研究延迟治疗的效果，将一组耳聋12周的动物植入模拟脑梗塞的刺激电极，然后接受胶质细胞源性神经营养因子(GDNF)为期4周的治疗。通过记录电诱发的ABR来测量SGN的电反应性。在最初的7周内，SGN迅速退化，表现为SGN数量的显著减少。变性过程随后放缓，7周和18周之间剩余神经元的数量没有差异。(C)2016年，作者神经科学研究杂志由Wiley期刊公司出版。
Today a cochlear implant (CI) may significantly restore auditory function, even for people with a profound hearing loss. Because the efficacy of a CI is believed to depend mainly on the remaining population of spiral ganglion neurons (SGNs), it is important to understand the timeline of the degenerative process of the auditory neurons following deafness. Guinea pigs were transtympanically deafened with neomycin, verified by recording auditory brainstem responses (ABRs), and then sacrificed at different time points. Loss of SGNs as well as changes in cell body and nuclear volume were estimated. To study the effect of delayed treatment, a group of animals that had been deaf for 12 weeks was implanted with a stimulus electrode mimicking a CI, after which they received a 4-week treatment with glial cell-derived neurotrophic factor (GDNF). The electrical responsiveness of the SGNs was measured by recording electrically evoked ABRs. There was a rapid degeneration during the first 7 weeks, shown as a significant reduction of the SGN population. The degenerative process then slowed, and there was no difference in the amount of remaining neurons between weeks 7 and 18. (c) 2016 The Authors Journal of Neuroscience Research Published by Wiley Periodicals, Inc.