Regions outside of the leucine-rich repeats of flax rust resistance proteins play a role in specificity determination
Regions outside of the leucine-rich repeats of flax rust resistance proteins play a role in specificity determination
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DOI：
10.1105/tpc.12.8.1367
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发表时间：
2000-08-01
期刊：
影响因子：
11.6
通讯作者：
Ellis, JG
中科院分区：
文献类型：
--
作者：
Luck, JE;Lawrence, GJ;Ellis, JG
在亚麻L基因座上存在多个控制不同基因对亚麻锈病抗性特异性的等位基因。在预测的蛋白质产物中可以识别至少三个不同的区域：Toll/白细胞介素-1受体同源性（TIR）区域、核苷酸结合位点（NBS）区域和富含亮氨酸的重复序列（LRR）区域。通过重组将L 6等位基因的TIR编码区替换为L2或LH的相应区域，将等位基因的特异性从L 6改变为L7。用L2或L9的等同区域替换L10的TIR和大部分NBS编码区产生具有新特异性的重组等位基因。然而，用L2的TIR编码区替换L10 TIR编码区产生了没有可检测特异性的等位基因。这些数据表明，非LRR区域可以确定等位基因产物之间的特异性差异，并且功能特异性涉及等位基因的蛋白质产物中的共适应多态性区域之间的相互作用。观察到TIR区域的多样化选择的行动的证据。
Multiple alleles controlling different gene-for-gene flax rust resistance specificities occur at the L locus of flax. At least three distinct regions can be recognized in the predicted protein products: the Toll/interleukin-1 receptor homology (TIR) region, a nucleotide binding site (NBS) region, and a leucine-rich repeat (LRR) region. Replacement of the TIR-encoding region of the L6 allele with the corresponding regions of L2 or LH by recombination changed the specificity of the allele from L6 to L7. Replacement of the TIR and most of the NBS-encoding region of L10 with the equivalent region of L2 or L9 generated recombinant alleles having a novel specificity. However, replacement of the L10 TIR-encoding region with the TIR-encoding region of L2 gave rise to an allele with no detectable specificity. These data indicate that non-LRR regions can determine specificity differences between allelic gene products and that functional specificity involves interactions between coadapted polymorphic regions in the protein products of the alleles. Evidence for the action of diversifying selection on the TIR region is observed.