Application of 23Na MRI to monitor chemotherapeutic response in RIF-1 tumors
Application of 23Na MRI to monitor chemotherapeutic response in RIF-1 tumors
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DOI：
10.1593/neo.05130
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发表时间：
2005-07-01
期刊：
影响因子：
4.8
通讯作者：
Bansal, N
中科院分区：
文献类型：
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作者：
Babsky, AM;Hekmatyar, SK;Bansal, N
通过体内Na-23和H-1磁共振成像（MRI）检查了烷化剂抗癌药物环磷酰胺（Cp）对皮下植入的放射诱导纤维肉瘤（RIF-1）肿瘤中Na-23信号强度（Na-23 SI）和水表观扩散系数（ADC）的影响。对未处理的对照组（n = 5）和Cp处理组（n = 6）C3 H小鼠进行MRI实验，在Cp注射（300 mg/kg）前进行一次，然后在处理后每天进行一次，持续3天。给药后2天和3天，给药动物的肿瘤体积显著降低。在相同的时间点，MRI实验显示在处理的肿瘤中Na-23 SI和水ADC两者都增加，而对照肿瘤没有显示任何显著变化。Na-23 SI和水ADC变化之间的相关性在CP处理组中显著增加，表明观察到的Na-23 SI和水ADC的增加是由相同的机制引起的。组织切片显示Na-23和水ADC SI增加区域的细胞密度降低。破坏性化学分析表明，Cp治疗增加了相对细胞外空间和肿瘤[Na+]。我们得出结论，水ADC和Na-23 SI的变化主要是由于细胞外23空间的增加。Na MRI和1H水ADC测量可能为监测化疗反应提供有价值的非侵入性技术。
Effects of an alkylating anticancer drug, cyclophosphamide (Cp), on Na-23 signal intensity (Na-23 SI) and water apparent diffusion coefficient (ADC) were examined in subcutaneously - implanted radiation-induced fibrosarcoma (RIF-1) tumors by in vivo Na-23 and H-1 magnetic resonance imaging (MRI). MRI experiments were performed on untreated control (n = 5) and Cp-treated (n = 6) C3H mice, once before Cp injection (300 mg/kg) then daily for 3 days after treatment. Tumor volumes were significantly lower in treated animals 2 and 3 days posttreatment. At the same time points, MRI experiments showed an increase in both Na-23 SI and water ADC in treated tumors, whereas control tumors did not show any significant changes. The correlation between Na-23 SI and water ADC changes was dramatically increased in the Cp-treated group, suggesting that the observed increases in Na-23 SI and water ADC were caused by the same mechanism. Histologic sections showed decreased cell density in the regions of increased Na-23 and water ADC SI. Destructive chemical analysis showed that Cp treatment increased the relative extracellular space and tumor [Na+]. We conclude that the changes in water ADC and Na-23 SI were largely due to an increase in extracellular 23 space. Na MRI and 1H water ADC measurements may provide valuable noninvasive techniques for monitoring chemotherapeutic responses.