The Caenorhabditis elegans schnurri homolog sma-9 mediates stage- and cell type-specific responses to DBL-1 BMP-related signaling
The Caenorhabditis elegans schnurri homolog sma-9 mediates stage- and cell type-specific responses to DBL-1 BMP-related signaling
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DOI：
10.1242/dev.00863
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发表时间：
2003-12-01
期刊：
影响因子：
4.6
通讯作者：
Savage-Dunn, C
中科院分区：
文献类型：
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作者：
Liang, J;Lints, R;Savage-Dunn, C
在秀丽隐杆线虫中，DBL-1通路，一种BMP/TGF β相关的信号级联，调节身体大小和雄性尾部发育。我们已经克隆了一个新的基因，sma-9，它编码线虫同源的Schnurri，一个大的锌指转录因子，调节dpp靶基因在果蝇。遗传相互作用、sma-9功能丧失表型和表达模式表明，sma-9作为下游组分，在DBL-1信号通路中是必需的，因此提供了Schnurri蛋白在BMP信号传导中保守作用的第一个证据。对SMA-9突变体表型的分析表明，SMA-9活性相对于已知的DBL-1途径组分在时间和空间上受到限制。与果蝇相比，多个选择性剪接的SMA-9转录本的存在表明蛋白质亚型具有潜在的不同细胞亚定位和分子功能。我们认为SMA-9亚型作为转录辅因子发挥作用，赋予DBL-1通路激活特异性反应。
In Caenorhabditis elegans, the DBL-1 pathway, a BMP/TGFbeta-related signaling cascade, regulates body size and male tail development. We have cloned a new gene, sma-9, that encodes the C elegans homolog of Schnurri, a large zinc finger transcription factor that regulates dpp target genes in Drosophila. Genetic interactions, the sma-9 loss-of-function phenotype, and the expression pattern suggest that sma-9 acts as a downstream component and is required in the DBL-1 signaling pathway, and thus provide the first evidence of a conserved role for Schnurri proteins in BMP signaling. Analysis of sma-9 mutant phenotypes demonstrates that SMA-9 activity is temporally and spatially restricted relative to known DBL-1 pathway components. In contrast with Drosophila schnurri, the presence of multiple alternatively spliced sma-9 transcripts suggests protein isoforms with potentially different cell sublocalization and molecular functions. We propose that SMA-9 isoforms function as transcriptional cofactors that confer specific responses to DBL-1 pathway activation.