Role of saccharides on lung preservation
Role of saccharides on lung preservation
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DOI：
10.1097/00007890-199907150-00021
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发表时间：
1999-07-15
期刊：
影响因子：
6.2
通讯作者：
Wada, H
中科院分区：
文献类型：
--
作者：
Fukuse, T;Hirata, T;Wada, H
背景。糖被认为是渗透性的，在缺血时可以作为器官的能量来源。然而，以往关于糖类对器官保存有效性的研究得出了相互矛盾的结果。我们比较了单糖（葡萄糖）、双糖（海藻糖、麦芽糖、蔗糖）和三糖（棉子糖）的保存作用，以研究糖对肺保存的作用是否取决于它们的分子量、能量水平维持和细胞保护作用。我们采用体外大鼠肺模型，以同种异体血液为灌注液。新鲜组冲洗后立即进行肺部再灌注。在另一组中，用含有葡萄糖、海藻糖、麦芽糖、蔗糖或棉子糖的溶液中的一种冲洗肺，并保存14小时。海藻糖组的结果与新鲜组相当。葡萄糖组、麦芽糖组和棉子糖组的分流分数、肺动脉压、与新鲜组和海藻糖组比较，呼吸压力峰值。两组间冲洗后肺总腺嘌呤核苷酸、三磷酸腺苷和保存水平均无差异。然而，再灌注后，葡萄糖、蔗糖、麦芽糖和棉子糖组的总腺嘌呤核苷酸水平显著降低。超微结构检查显示葡萄糖组、蔗糖组、麦芽糖组和棉子糖组内皮细胞损伤。这些结果表明，糖的作用可能取决于它们的细胞保护作用，而不是取决于保存的肺的不必要的活动或能量水平维持。海藻糖被证明优于其他糖类。
Background. Saccharides are considered to play a role as osmotic impermeants and serve as an energy source for the organ during ischemia. However, previous studies on the effectiveness of saccharides on organ preservation have yielded conflicting results. We compared the preservative effects of a monosaccharide (glucose), disaccharides (trehalose, maltose, sucrose), and a trisaccharide (raffinose) to investigate whether the effects of saccharides on lung preservation depend on their molecular weight, energy-level maintenance, and cytoprotective effects.Methods. We used an ex vivo rat lung model using homologous blood as the perfusate. In the fresh group, the lungs were reperfused immediately after flush. In the other groups, the lungs were flushed with one of the solutions containing glucose, trehalose, maltose, sucrose, or raffinose and preserved for 14 hours.Results. The results of the trehalose group were comparable to those of the fresh group. The glucose, maltose, and raffinose groups showed significantly higher levels of shunt fraction, pulmonary artery pressure,. and peak inspiratory pressure compared with the fresh and trehalose groups. There were no differences among the groups in the levels of total adenine nucleotides, adenosine triphosphate of the lung after flush, and preservation. However, after reperfusion, levels of total adenine nucleotides became significantly lower in the glucose, sucrose, maltose, and raffinose groups. Ultrastructural examination revealed endothelial cell injury in the glucose, sucrose, maltose, and raffinose groups.Conclusions. These results show that the effects of saccharides may depend on their cytoprotective effect rather than on impermeant activity or energy-level maintenance of the preserved lung. Trehalose proved to be superior to the other saccharides.