Reevaluation of area 3b in the cat based on architectonic and electrophysiological studies: regional variability with functional and anatomical consistencies.
Reevaluation of area 3b in the cat based on architectonic and electrophysiological studies: regional variability with functional and anatomical consistencies.
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根据结构和电生理学研究重新评估猫的 3b 区：区域变异性与功能和解剖学一致性。
DOI：
10.1002/cne.903410307
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Salimi,I
中科院分区：
文献类型：
--
作者：
Leclerc,SS;Avendano,C;Dykes,RW;Waters,RS;Salimi,I
大多数对猫初级躯体感觉皮层的解剖学和电生理学研究都依赖于Hassler和Muhs-Clement的（J. Hirnforsch. 6：377 - 420，1964）区分3a区和3b区的细胞和骨髓结构描述;然而，在已发表的研究中，这些区域的划定存在差异，表明许多工作者发现很难系统地应用这些标准。我们研究了尼氏染色矢状切片中3b区的细胞结构，在处死前从该切片中获得了电生理数据。在脑回的不同中外侧位置放置电极穿透的头尾侧行，定位区域对皮肤或深部结构的刺激做出反应。小电解病变允许这些数据与细胞结构。一项对躯干和四肢代表的系统研究发现，皮质区域的皮肤反应以厚且细胞密集的颗粒层IV为特征，然而，这些相同区域在层V中具有可变的中型和/或大型锥体细胞群。锥体细胞几乎不存在于前肢代表中，但在躯干和后肢代表中存在不同程度的锥体细胞。此外，第四层的相对厚度和细胞密度在前肢中比在后肢中更大。深层反应被发现在皮层特征在于由一个较薄的层IV。由于3a区V层的特性是可变的，因此它对确定3a区和3b区之间的边界用处不大。乙酰胆碱酯酶染色的强度和层状分布的明显变化发生在区域3a和3b之间，使其成为尼氏染色的有用辅助。
Most anatomical and electrophysiological studies of the cat primary somatosensory cortex rely on Hassler and Muhs‐Clement's (J. Hirnforsch. 6:377‐420, 1964) cyto‐ and myeloarchitectonic description distinguishing area 3a from area 3b; however, discrepancies in the delineation of these areas in published studies suggest that many workers have found it difficult to apply those criteria systematically. We examined the cytoarchitecture of area 3b in Nissl stained sagittal sections from which electrophysiological data had been obtained prior to sacrifice. Rostrocaudal rows of electrode penetrations placed at different mediolateral positions in the gyrus located regions responsive to stimulation of either cutaneous or deep structures. Small electrolytic lesions allowed these data to be related to the cytoarchitecture. A systematic study throughout the trunk and limb representations found cutaneous responses in cortical regions characterized by a thick and cell‐dense granular layer IV, however these same regions had a variable population of medium‐sized and/or large pyramidal cells in layer V. Pyramidal cells were practically absent from the forelimb representation, but were present to varying degrees in the trunk and hindlimb representations. Moreover, the relative thickness and cell‐density in layer IV were greater in the forelimb than in the hindlimb representations. Deep responses were found in cortex characterized by a thinner layer IV. Since the characteristics of layer V in area 3a were variable, it was less useful for identification of the border between areas 3a and 3b. Clear changes in the intensity and laminar distribution of acetylcholinesterase staining occurred between areas 3a and 3b, making this a useful adjunct to the Nissl stain.