Muscarinic signaling in ciliated tracheal epithelial cells: dual effects on Ca2+ and ciliary beating.
Muscarinic signaling in ciliated tracheal epithelial cells: dual effects on Ca2+ and ciliary beating.
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纤毛气管上皮细胞中的毒蕈碱信号传导：对 Ca2+ 和纤毛跳动的双重影响。
DOI：
10.1152/ajplung.1997.272.2.l301
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Bookman,RJ
中科院分区：
文献类型：
--
作者：
Salathe,M;Lipson,EJ;Ivonnet,PI;Bookman,RJ
研究胆碱能信号转导通路，调节纤毛搏动频率（CBF），培养的绵羊气管上皮细胞成像相衬（CBF）和荧光（Ca 2+）显微镜技术的组合。在单个细胞中，乙酰胆碱（ACh）瞬时增加CBF和细胞内Ca 2+浓度（[Ca 2 +]i），主要是通过从内部存储的Ca 2+释放，与Ca 2+内流的小延迟贡献。尼古丁激动剂没有改变CBF或[Ca 2 +]i，而阿托品阻断乙酰胆碱刺激的瞬变，与毒蕈碱受体的参与一致。4-二苯基乙酰氧基-N-甲基哌啶甲碘化物在抑制乙酰胆碱诱导的[Ca 2 +]i峰方面的效力比哌仑西平强约100倍，表明该受体是一种非特异性（M3）亚型。有趣的是，在通过毒胡萝卜素耗尽细胞内Ca 2+储存后，ACh引起CBF和[Ca 2 +]i的快速瞬时降低，再次具有M3受体的拮抗剂特征。我们的结论是，激活M3毒蕈碱受体启动特定的信号通路，同时采取行动，以增加和减少[Ca 2 +]i和CBF。
To examine cholinergic signal transduction pathways that modulate ciliary beat frequency (CBF), cultured ovine tracheal epithelial cells were imaged using a combination of phase-contrast (CBF) and fluorescence (Ca2+) microscopy techniques. In single cells, acetylcholine (ACh) transiently increased CBF and intracellular Ca2+ concentration ([Ca2+]i), mainly by Ca2+ release from internal stores, with a small delayed contribution from Ca2+ influx. Nicotinic agonists did not alter CBF or [Ca2+]i, whereas atropine blocked the ACh-stimulated transients, consistent with the involvement of muscarinic receptors. 4-Diphenylacetoxy-N-methylpiperidine methiodide was approximately 100 times more potent than pirenzepine in inhibiting the ACh-induced [Ca2+]i peaks, suggesting that the receptor is a pharmacologically defined (M3) subtype. Interestingly, after depletion of intracellular Ca2+ stores by thapsigargin, ACh caused a rapid transient decrease in both CBF and [Ca2+]i, again with an antagonist profile of M3 receptors. We conclude that activation of M3 muscarinic receptors initiates specific signaling pathways that act simultaneously to increase and decrease [Ca2+]i and CBF.