Aptamer-Mediated Surface-Enhanced Raman Spectroscopy Intensity Amplification
Aptamer-Mediated Surface-Enhanced Raman Spectroscopy Intensity Amplification
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DOI：
10.1021/nl102495j
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发表时间：
2010-10-01
期刊：
影响因子：
10.8
通讯作者：
Moskovits, Martin
中科院分区：
文献类型：
--
作者：
Kim, Nam Hoon;Lee, Seung Joon;Moskovits, Martin
一个双功能腺苷敏感的双链DNA适体被用于在体积金表面和通过生物素-亲和素连接的金纳米颗粒（Au NP）之间创建和控制表面增强拉曼光谱（SERS）热点。Au NP被拉曼报告分子4-氨基苯基硫醇（4-ABT）修饰在靶分子腺苷存在下，4- abt的SERS谱受（浓度依赖）因子的影响强度增加了4倍，原位原子力显微镜成像显示含Au np的适体的平均高度降低了约5 nm，这与观察到的SERS强度变化一致。因为适体的几何变化是由一个或两个分子引起的，而由此产生的SERS强度变化涉及驻留在修饰热点的许多报告分子。适体放大了SERS效应
A bifunctional adenosine-sensitive double-stranded DNA aptamer was used to create and control a surface-enhanced Raman spectroscopy (SERS) hot spot between a bulk Au surface and a gold nanoparticle (Au NP) attached to the apramer via a biotin-avidin linkage The Au NP was decorated with 4-aminobenzenethiol (4-ABT), a Raman reporter molecule In the presence of adenosine, the target molecule, the SERS spectrum of 4-ABT increased in intensity by (concentration-dependent) factors as large as similar to 4, in situ, atomic force microscopy imaging showed the mean height of the Au NP-bearing aptamer to decrease by similar to 5 nm consistent with the observed SERS intensity change Because the aptamer's geometrical change is induced by one or two molecules, while the resulting SERS intensity changes involve many reporter molecules residing in the modified hot spot, the aptamer amplifies the SERS effect