The influence of surface energy on competitive protein adsorption on oxidized NiTi surfaces
The influence of surface energy on competitive protein adsorption on oxidized NiTi surfaces
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DOI：
10.1016/j.biomaterials.2006.09.040
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发表时间：
2007-02-01
期刊：
影响因子：
14
通讯作者：
Gil, Javier
中科院分区：
文献类型：
--
作者：
Michiardi, Alexandra;Aparicio, Conrado;Gil, Javier
对NiTi形状记忆合金表面，未经处理和通过新的氧化处理（OT）氧化以获得无ni表面进行了表面能和蛋白质吸附行为的比较。确定了表面能的极性和色散分量。纤维连接蛋白和白蛋白之间的竞争吸附过程进行了i -125放射性标记。此外，两种蛋白质和NiTi表面之间的粘附强度已通过进行洗脱测试进行评估。结果表明，OT处理通过显著提高表面能的极性组分，增强了NiTi表面的亲水性。此外，OT处理增加了纤维连接蛋白和白蛋白的吸附量。它还增加了纤维连接蛋白对镍钛表面的亲和力。洗脱试验结果表明，纤维连接蛋白的构象变化是NiTi材料化学成分和表面处理的函数。最后，在吸附白蛋白的量和镍钛表面能的极性组分之间的线性关系已经被证明。这项工作表明，OT处理会影响NiTi材料的表面能值，进而影响蛋白质的吸附过程。(c) 2006 Elsevier Ltd.版权所有。
NiTi shape memory alloy surfaces, untreated, and oxidized by a new oxidation treatment (OT) in order to obtain a Ni-free surface, have been compared in terms of surface energy and protein adsorption behavior. The polar and dispersive components of the surface energy have been determined. A competitive adsorption process between fibronectin and albumin has been carried out by I-125-radiolabeling. Moreover, the adhesion strength between both proteins and NiTi surfaces has been evaluated by performing an elution test. The results show that the OT treatment enhances the hydrophilic character of NiTi surfaces by significantly increasing the polar component of their surface energy. Moreover, the OT treatment increases the amount of fibronectin and albumin adsorbed. It also increases the fibronectin affinity for NiTi surfaces. The elution test results could suggest a conformational change of fibronectin as a function of chemical composition of NiTi material and of surface treatment. Finally, a linear correlation between the amount of adsorbed albumin and the polar component of the surface energy of NiTi surfaces has been demonstrated. This work indicates that the OT treatment has an influence on the surface energy value of NiTi materials, which in turn influences the protein adsorption process. (c) 2006 Elsevier Ltd. All rights reserved.