A large open reading frame (orf1995) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein
A large open reading frame (orf1995) in the chloroplast DNA of Chlamydomonas reinhardtii encodes an essential protein
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DOI：
10.1007/s004380050368
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发表时间：
1997-02-20
期刊：
影响因子：
--
通讯作者：
Leu, S
中科院分区：
文献类型：
--
作者：
Boudreau, E;Turmel, M;Leu, S
在莱茵衣藻的叶绿体基因组中发现了一个编码1995个氨基酸的蛋白质的开放阅读框(Orf1995)。除了有一个短的疏水N-末端结构域和五个假定的跨膜螺旋外，预测的orf1995产物是高度碱性的。Orf1995可能是陆地植物中ycf1基因的同源基因，其功能尚未确定。用orf1995基因中断转化的莱茵梭菌突变株对该基因的野生型和中断等位基因保持异质性，表明orf1995产物是细胞生存所必需的。
An open reading frame potentially encoding a protein of 1995 amino acids (orf1995) has been found in the chloroplast genome of the green alga Chlamydomonas reinhardtii. Besides having a short hydrophobic N-terminal domain with five putative transmembrane helices, the predicted orf1995 product is highly basic. orf1995 might be a homologue of the ycf1 gene in land plants, whose function has not yet been determined. Mutants of C. reinhardtii transformed with a disruption of orf1995 remain heteroplasmic for the wild-type and disrupted alleles of this gene, indicating that the orf1995 product is essential for cell survival.