High density culture of anchorage-dependent animal cells by polyurethane foam packed-bed culture systems
High density culture of anchorage-dependent animal cells by polyurethane foam packed-bed culture systems
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聚氨酯泡沫填充床培养系统高密度培养贴壁依赖性动物细胞
DOI：
10.1007/bf00184680
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发表时间：
1991
影响因子：
5
通讯作者：
K. Funatsu
中科院分区：
文献类型：
--
作者：
T. Matsushita;Hiromichi Hidaka;Ken;Y. Kawakubo;K. Funatsu
猴肾细胞（Vero）和中国仓鼠卵巢细胞（CHO-K1）贴附于聚氨酯泡沫塑料（PUF）内表面，在PUF平板填充床培养系统中生长至高细胞密度（分别为1.1 × 108个/cm ~ 3 PUF和4.2 × 107个/cm ~ 3 PUF）。该Vero细胞密度是先前用PUF颗粒填充床培养系统获得的密度的两倍。用PUF-圆盘填充床培养Vero细胞，细胞密度最高可达6.7 × 107个/cm ~ 3培养皿容积。根据在PUF表面和培养皿上以单层生长的CHO-K1的细胞密度（每单位体积的PUF），我们估计了对细胞生长有效的PUF板的表面积与体积比为约109 cm 2/cm 3。
Monkey kidney cells (Vero) and Chinese hamster ovary cells (CHO-K1) attached to the internal surface of polyurethane foam (PUF) and grew to a high cell density (1.1 × 108cells/cm3PUF and 4.2 × 107cells/cm3PUF, respectively) in a PUF-plates packed-bed culture system. This density of Vero cells was twice that obtained previously with a PUF-particles packed-bed culture system. A maximum cell density of 6.7 × 107cells/cm3culture vessel volume was obtained in a PUF-disc packed-bed culture of Vero cells. From the cell density of CHO-K1, growing in a monolayer on the surface of PUF and a petri dish, per bulk volume of PUF, we estimated that a surface area to volume ratio of PUF plates effective for cell growth was about 109 cm2/cm3.