Determination of the order of gene function in the yeast nuclear division pathway using cs and ts mutants.
Determination of the order of gene function in the yeast nuclear division pathway using cs and ts mutants.
复制标题
使用 cs 和 ts 突变体确定酵母核分裂途径中基因功能的顺序。
DOI：
10.1093/genetics/100.4.565
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发表时间：
1982
期刊：
影响因子：
3.3
通讯作者：
Botstein,D
中科院分区：
文献类型：
--
作者：
Moir,D;Botstein,D
利用冷敏感（cs）和热敏感（ts）条件致死突变特异性影响出芽酵母（Saccharomyces cerevisiae）的细胞分裂周期来确定基因功能的顺序。使用cs- ts双突变体的互变温度实验揭示了执行点和突变表型非常相似的基因之间的详细功能顺序。这些数据表明，整个细胞周期途径的核分支本身至少包含一个分支。
Cold-sensitive (cs) and heat-sensitive (ts) conditional-lethal mutations that affect specifically the cell division cycle of budding yeast (Saccharomyces cerevisiae) were used to determine the order of gene function. Reciprocal temperature-shift experiments usingcs-tsdouble mutants revealed a detailed order of function among genes whose execution points and mutant phenotypes are very similar. The data suggest that the nuclear branch of the overall cell-cycle pathway itself contains at least one branch.