Exogenous calcium alleviates cadmium-induced oxidative stress in rice (Oryza sativa L.) seedlings by regulating the antioxidant defense and glyoxalase systems
Exogenous calcium alleviates cadmium-induced oxidative stress in rice (Oryza sativa L.) seedlings by regulating the antioxidant defense and glyoxalase systems
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DOI：
10.1007/s40415-015-0240-0
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发表时间：
2016-06
影响因子：
1.6
通讯作者：
Anisur Rahman;M. Mostofa;K. Nahar;M. Hasanuzzaman;M. Fujita
中科院分区：
文献类型：
--
作者：
Anisur Rahman;M. Mostofa;K. Nahar;M. Hasanuzzaman;M. Fujita
本研究旨在探讨外源钙对水稻抗氧化防御系统和谷胱甘肽酶系统在镉胁迫下的调节作用。水培生长14天的水稻（Oryza sativa L.）CV.将BRRI dhan 29）幼苗暴露于单独的0.25和0.5mM CdCl 2以及与2.5mM CaCl 2组合3天。暴露于镉引起褪绿，卷叶症状，和生长抑制。在生长介质中的较高浓度的镉导致较高的镉积累，诱导氧化应激通过过量生产的活性氧（ROS）通过破坏抗氧化防御系统。镉处理增加了甲基乙二醛（MG）水平。在镉处理的生长培养基中补充钙减少镉的吸收。钙的施用也显著增加了抗氧化系统中的超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽S-转移酶（GST）、单脱氢抗坏血酸还原酶（MDHAR）和脱氢抗坏血酸还原酶（达尔）的活性增加，两种浓度Cd处理均提高了水稻幼苗谷胱甘肽酶系统中的谷胱甘肽酶I（Gly I）和谷胱甘肽酶II（Gly II）活性。外源钙的应用调节抗氧化防御和glycoprotein酶系统，逆转过度产生的ROS和解毒MG，这反过来又降低镉的毒性。
The present study was undertaken to investigate the regulatory role of exogenous application of calcium (Ca) in enhancing the antioxidant defense and glyoxalase systems in mitigating cadmium (Cd) stress in rice. Hydroponically grown 14-day-old rice (Oryza sativaL. cv. BRRI dhan29) seedlings were exposed to 0.25 and 0.5 mM CdCl2alone and in combination with 2.5 mM CaCl2for 3 days. Exposure to Cd caused chlorosis, leaf rolling symptoms, and growth inhibition. A higher concentration of Cd in the growth medium resulted in higher Cd accumulation, which induced oxidative stress through overproduction of reactive oxygen species (ROS) by disrupting the antioxidant defense system. Cadmium treatment increased the methylglyoxal (MG) level. Calcium supplementation in the Cd-treated growth medium reduced Cd uptake. Application of Ca also significantly increased the (ascorbate) AsA content, increased the activities of superoxide dismutase (SOD), catalase (CAT), glutathioneS-transferase (GST), monodehydroascorbate reductase (MDHAR), and dehydroascorbate reductase (DHAR) in the antioxidant system, and increased the glyoxalase I (Gly I) and glyoxalase II (Gly II) activities in the glyoxalase system in rice seedlings exposed to both levels of Cd. Exogenous Ca application regulated the antioxidant defense and glyoxalase systems, which reversed overproduced ROS and detoxified MG, which in turn reduced Cd toxicity.