Isolation and characterization of tri- and tetra-repeat microsatellite loci in the white-spotted charr Salvelinus leucomaenis (Salmonidae)
Isolation and characterization of tri- and tetra-repeat microsatellite loci in the white-spotted charr Salvelinus leucomaenis (Salmonidae)
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白斑红点鲑 Salvelinus leucomaenis（鲑科）三重复和四重复微卫星位点的分离和表征
DOI：
10.1111/jfb.12628
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发表时间：
2015
影响因子：
2
通讯作者：
Yamamoto S. Sekino M.
中科院分区：
文献类型：
--
作者：
Shoichiro Yamamoto;Hitoshi Kubota;Koh Hasegawa;Tomoyuki Nakamura;Yamamoto S. Sekino M.
为白色斑点红点鲑（Salvelinus leucomaenis）开发了三基序重复和四基序重复微卫星标记位点。454焦磷酸测序用于发现重复序列，并确定了8个微卫星引物组，可用于估计多态性。野生群体的等位基因数为2 ~ 4个，观察杂合度为0.180 ~ 0.600，期望杂合度为0.188 ~ 0.599。
Tri‐ and tetra‐motif repeat microsatellite marker loci were developed for the white‐spotted charrSalvelinus leucomaenis. The 454 pyrosequencing was used to discover repeat arrays, and eight microsatellite‐primer sets, available for the estimation of polymorphisms, were identified. The number of alleles in a wild population ranged from two to four and the observed and expected heterozygosities were 0·180–0·600 and 0·188–0·599, respectively.