CPEB3 regulates the proliferation and apoptosis of bovine cumulus cells.
CPEB3 regulates the proliferation and apoptosis of bovine cumulus cells.
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DOI：
10.1111/asj.13416
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发表时间：
2020-01
期刊：
影响因子：
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通讯作者：
Ying Wang;Chengzhen Chen;Xu-huang Fu;Jian-Bo Liu;Yanxia Peng;Yi-jie Wang;Dong-Xu Han;Zhe Zhang;Bao Yuan;Yan Gao;Hao Jiang;Jiabao Zhang
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文献类型：
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作者：
Ying Wang;Chengzhen Chen;Xu-huang Fu;Jian-Bo Liu;Yanxia Peng;Yi-jie Wang;Dong-Xu Han;Zhe Zhang;Bao Yuan;Yan Gao;Hao Jiang;Jiabao Zhang
胞质多聚腺苷酸化元件结合蛋白3（CPEB 3）是胞质多聚腺苷酸化元件结合蛋白家族的一员，在非洲爪蟾卵母细胞中被发现可调节休眠和掩蔽mRNA的翻译，并在调节细胞和组织的生物学功能中发挥潜在的作用。然而，其在卵丘细胞中的作用尚不清楚。本研究用小分子干扰RNA（siRNA）抑制牛卵丘细胞中CPEB 3 mRNA的表达。在抑制CPEB 3后测量细胞周期进展、增殖和凋亡。随后，检测细胞内活性氧含量、线粒体膜电位和扩增相关基因表达的变化。结果表明，CPEB 3抑制后，卵丘细胞出现细胞周期异常，S期和G2/M期细胞明显增多，细胞增殖增强，凋亡率降低。这些效应可能是由于CPEB 3抑制诱导的细胞内活性氧水平降低;线粒体膜电位升高;细胞凋亡减少; CCNA、CCND、CCNE、CDK 2、CDK 4、CDK 6、p21和p27 mRNA表达下调;以及CCNB、CDK 1、HAS 2、PTGS 2、PTX 3和CEBPB mRNA表达上调。因此，CPEB 3在调节牛卵丘细胞的生物学和生理功能方面具有潜在的作用。
Cytoplasmic polyadenylation element-binding protein 3 (CPEB3) is a member of the Cytoplasmic polyadenylation element-binding family, which has been found to regulate the translation of dormant and masked mRNA in Xenopus oocytes and plays potential roles in regulating biological functions in cells and tissues. However, its role in cumulus cells is not clear. In this study, the mRNA expression of CPEB3 in bovine cumulus cells was inhibited with small interfering RNA. Cell cycle progression, proliferation, and apoptosis were measured after inhibition of CPEB3. Subsequently, changes in intracellular Reactive oxygen species content, mitochondrial membrane potential and expansion-related gene expression were examined. The results showed that after CPEB3 inhibition, cumulus cells had an abnormal cell cycle, the numbers of cells in the S and G2/M phases were significantly increased, cell proliferation was increased and apoptosis rates were decreased. These effects were likely due CPEB3 inhibition-induced decreases in intracellular Reactive oxygen species levels; increases in mitochondrial membrane potential; decreases in apoptosis; downregulation of CCNA, CCND, CCNE, CDK2, CDK4, CDK6, p21, and p27 mRNA expression; and upregulation of CCNB, CDK1, HAS2, PTGS2, PTX3, and CEBPB mRNA expression. Therefore, CPEB3 plays potential roles in regulating the biological and physiological functions of bovine cumulus cell.