Paradoxical expression of IL-28B mRNA in peripheral blood in human T-cell leukemia virus type-1 mono-infection and co-infection with hepatitis C virus.
Paradoxical expression of IL-28B mRNA in peripheral blood in human T-cell leukemia virus type-1 mono-infection and co-infection with hepatitis C virus.
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DOI：
10.1186/1743-422x-9-40
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发表时间：
2012-02-15
期刊：
影响因子：
4.8
通讯作者：
Nakao K
中科院分区：
文献类型：
--
作者：
Kamihira S;Usui T;Ichikawa T;Uno N;Morinaga Y;Mori S;Nagai K;Sasaki D;Hasegawa H;Yanagihara K;Honda T;Yamada Y;Iwanaga M;Kanematu T;Nakao K
已知人类t细胞白血病病毒1型（HTLV-1）携带者同时感染丙型肝炎病毒（HCV），其相关疾病的风险高于单一感染者。最近的研究表明，IL-28B多态性rs8099917不仅与IFN对HCV的治疗反应有关，还与先天免疫和抗病毒活性有关。我们的研究目的是阐明IL-28B基因多态性（rs8099917）是否与HTLV-1/HCV合并感染相关。340例个体的基因分型和病毒血清学分析表明，rs8099917 SNP的IL-28B基因型分布在不同病毒感染状态下无显著差异。然而，HTLV-1单一感染的IL-28B mRNA表达水平比HTLV-1/HCV合并感染高3.8倍。高表达与TT相关（OR, 6.25），低表达与两种病毒共感染相关（OR, 9.5）。然而，下调与ATL发展之间没有关联（OR, 0.8）。HTLV-1单感染以基因型依赖的方式上调IL-28B转录本的表达，而HTLV-1/HCV联合感染无论是否发生ATL，均下调IL-28B转录本的表达。
Human T-cell leukemia virus type-1 (HTLV-1) carriers co-infected with and hepatitis C virus (HCV) have been known to be at higher risk of their related diseases than mono-infected individuals. The recent studies clarified that IL-28B polymorphism rs8099917 is associated with not only the HCV therapeutic response by IFN, but also innate immunity and antiviral activity. The aim of our research was to clarify study whether IL-28B gene polymorphism (rs8099917) is associated with HTLV-1/HCV co-infection. The genotyping and viral-serological analysis for 340 individuals showed that IL-28B genotype distribution of rs8099917 SNP did not differ significantly by respective viral infection status. However, the IL-28B mRNA expression level was 3.8 fold higher in HTLV-1 mono-infection than HTLV-1/HCV co-infection. The high expression level was associated with TT (OR, 6.25), whiles the low expression was associated with co-infection of the two viruses (OR, 9.5). However, there was no association between down-regulation and ATL development (OR, 0.8). HTLV-1 mono-infection up-regulates the expression of IL-28B transcripts in genotype-dependent manner, whiles HTLV-1/HCV co-infection down-regulates regardless of ATL development.