Cell Surface Deformation during an Action Potential
Cell Surface Deformation during an Action Potential
复制标题
DOI：
10.1016/j.bpj.2017.11.3776
复制
发表时间：
2018-01-23
影响因子：
3.4
通讯作者：
Schneider, Matthias F.
中科院分区：
文献类型：
--
作者：
Fillafer, Christian;Mussel, Matan;Schneider, Matthias F.
许多细胞和组织的兴奋与细胞力学变化有关。本文提出的证据证实，单个细胞在动作电位期间变形。结果表明，植物细胞（Chara braunii internodes）的兴奋伴随着细胞表面在微米范围内（类似于1-10 μ m）的面外位移。细胞变形的发生与动作电位的去极化相一致。机械脉冲：1)以与电脉冲相同的速度传播（在实验精度范围内，类似于10毫米每秒（-1）），2)可逆，3)在大多数情况下具有双相性质（152个实验中的109个），4)可能独立于肌动蛋白-肌球蛋白运动。微移管吸吸实验证实了细胞皮层存在瞬时力学变化。理论分析表明，这种现象可以用细胞表面机械性能（跨膜压力、表面张力和弯曲刚度）的可逆变化来解释。综上所述，这些发现对正在进行的关于细胞兴奋性的物理性质的争论有所贡献。
The excitation of many cells and tissues is associated with cell mechanical changes. The evidence presented herein corroborates that single cells deform during an action potential. It is demonstrated that excitation of plant cells (Chara braunii internodes) is accompanied by out-of-plane displacements of the cell surface in the micrometer range (similar to 1-10 mu m). The onset of cellular deformation coincides with the depolarization phase of the action potential. The mechanical pulse: 1) propagates with the same velocity as the electrical pulse (within experimental accuracy, similar to 10 mm s(-1)), 2) is reversible, 3) in most cases is of biphasic nature (109 out of 152 experiments), and 4) is presumably independent of actin-myosin-motility. The existence of transient mechanical changes in the cell cortex is confirmed by micropipette aspiration experiments. A theoretical analysis demonstrates that this observation can be explained by a reversible change in the mechanical properties of the cell surface (transmembrane pressure, surface tension, and bending rigidity). Taken together, these findings contribute to the ongoing debate about the physical nature of cellular excitability.