Tuberculosis transmission in Northwest of Iran: Using MIRU-VNTR, ETR-VNTR and IS6110-RFLP methods
Tuberculosis transmission in Northwest of Iran: Using MIRU-VNTR, ETR-VNTR and IS6110-RFLP methods
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DOI：
10.1016/j.meegid.2010.09.013
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发表时间：
2011-01-01
影响因子：
3.2
通讯作者：
Hanifi, Gholam Reza
中科院分区：
文献类型：
--
作者：
Asgharzadeh, Mohammad;Kafil, Hossein Samadi;Hanifi, Gholam Reza
结核病是成年人死于单一传染病的主要原因，每年约有200万人死于结核病。确定传播感染源对于减少结核病是必要的。在这项研究中，我们使用IS6110-RFLP、MIRU-VNTR和ETR-VNTR方法来确定2004年3月至2005年3月伊朗西北部结核病的传播动态。共收集培养阳性菌株156株，其中Orumieh菌株51株，Tabriz菌株105株。用标准化方法进行IS6110-RFLP，用聚合酶链式反应对12个Miru-VNTR基因座进行Miru-VNTR扩增，对ETR-A、ETR-B和ETR-C进行ETR扩增。利用MVSP软件和MIRU-VNTRplus网站进行遗传关系分析。结果表明，菌株的16110个拷贝数在0~17之间。IS6110-RFLP分析得到107条独特图谱和16条共同图谱，MIRU-VNTR116条清晰可见，其中21条为聚类图谱，95条为独特图谱。结合Miru-VNTR和ETR-VNTR数据，我们增加了分型方法的区分力，从而识别出25个类群。MIRU和ETR-A的10、26、40基因座具有很强的区分性。根据MIRU-VNTRplus数据库的初步数据，本研究中的血统分布为多匹配9.09%，牛群9.74%，S 8.44%，新-1 0.65%，乌干达10.65%，哈勒姆0.65%，喀麦隆0.65%，X 0.65%。IS6110-RFLP对结核病传播所占比例的最低估计值为20.1%，而MIRU和ETR的最低估计值为18.8%。总而言之，由于结核病传播的最低估计，伊朗西北部的结核病卫生状况令人满意，结核病的重新激活导致了80%以上的新增结核病。根据IS6110-RFLP方法的结果和缺点，我们强烈推荐Miru-VNTRplus ETR-VNTR是一种简单、可靠、重复性好、分辨率高的POWER方法。皇冠版权所有(C)2010由爱思唯尔出版。保留所有权利。
Tuberculosis is the main cause for adults' death from a single infectious agent killing about 2 million people every year. Identifying the source of transmission infection is necessary to decrease tuberculosis. In this study we used IS6110-RFLP, MIRU-VNTR and ETR-VNTR methods to determine transmission dynamic of tuberculosis in Northwest of Iran from March 2004 to March 2005. 156 culture positive isolates (51 from Orumieh and 105 from Tabriz) were collected. IS6110-RFLP was done by standardized method and MIRU-VNTR was done by 12 primers specific for 12 MIRU-VNTR loci by polymerase chain reaction and ETR was carried out by primers specific for ETR-A, ETR-B and ETR-C as well. Genetic relationship analyses were performed by MVSP software and MIRU-VNTRplus website. The results revealed that the copy number of 16110 in isolates ranged from 0 to 17. One hundred and seven unique patterns and 16 shared patterns by 2-10 isolates were determined by IS6110-RFLP, and 116 distinct patterns were identified by MIRU-VNTR including 21 clustered patterns and 95 unique patterns. Having combined MIRU-VNTR and ETR-VNTR data, we increased the discriminatory power of typing method, thus 25 clusters were identified. Loci 10, 26, 40 in MIRU and ETR-A were highly discriminatory. Distribution of lineages in our study according to tentative data in MIRU-VNTRplus database was multi-matches 9.09%, Bovis 9.74%, S 8.44%, New-1 0.65%, Uganda 10.65%, Haarlem 0.65%, Cameroon 0.65% and X 0.65%. The minimum estimate for tuberculosis proportion due to transmission was 20.1% with IS6110-RFLP and was 18.8% with MIRU and ETR. In conclusion, tuberculosis sanitation status in northwest of Iran due to minimum estimate of tuberculosis transmission was satisfactory, and the reactivation of tuberculosis was responsible for more than 80% of new tuberculosis. According to the results and drawbacks of IS6110-RFLP method we strongly recommend MIRU-VNTRplus ETR-VNTR as an easy, reliable, reproducible and highly discriminatory power method. Crown Copyright (C) 2010 Published by Elsevier B.V. All rights reserved.