Aged-engineered nanoparticles effect on sludge anaerobic digestion performance and associated microbial communities
Aged-engineered nanoparticles effect on sludge anaerobic digestion performance and associated microbial communities
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DOI：
10.1016/j.scitotenv.2017.07.178
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发表时间：
2017-12-31
影响因子：
9.8
通讯作者：
Coulon, Frederic
中科院分区：
文献类型：
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作者：
Eduok, Samuel;Ferguson, Robert;Coulon, Frederic
为了研究老化的工程纳米粒子 (a-ENP) 对污泥消化性能的潜在影响，向 150 L 中试厌氧消化器 (AD) 注入初级活性污泥和废活性污泥的混合物，并掺入氧化银、二氧化钛和氧化锌的混合物或其等效散装金属盐的混合物，以达到 250、2000 和 2800 mg 的目标浓度 分别为kg(-1)干重。加标消化池中的挥发性脂肪酸 (VEA) 高出 1.2 倍，与对照条件显着不同 (p = 0.05)。具体而言，与加标消化器相比，对照消化器中的异戊酸浓度低 2 倍，而加标 ENP 消化器中的硫化氢浓度低 2 倍，表明对硫酸盐还原微生物有抑制作用。根据醚连接的类异戊二烯浓度，添加 ENP 的消化器中产甲烷菌的总丰度比对照和添加金属盐的消化器低 1A 倍。焦磷酸测序表明，与对照消化器相比，添加 ENP 的消化器中产甲烷菌的丰度和多样性降低了 80%。 Methanosurcina acetivoruns 和 Methunosurcina barkeri 被认为具有纳米耐受性，因为与其他消化器相比，它们的相对丰度分别增加了 6 倍和 11 倍。研究结果进一步为梭杆菌放线菌的恢复力和 ENP 的特洛伊木马效应提供了令人信服的证据，ENP 为某些生物体提供了竞争优势，同时降低了微生物丰度和多样性。 (C) 2017 年作者。由 Elsevier B.V 出版。这是一篇基于 CC BY 许可证 (littp://creativecommons.org/licenses/by/4.0/) 的开放获取文章。
To investigate the potential effect of aged engineered nanoparticles (a-ENPs) on sludge digestion performance, 150 L pilot anaerobic digesters (AD) were fed with a blend of primary and waste activated sludge spiked either with a mixture of silver oxide, titanium dioxide and zinc oxide or a mixture of their equivalent bulk metal salts to achieve a target concentration of 250,2000, and 2800 mg kg(-1) dry weight, respectively. Volatile fatty acids (VEA) were 1.2 times higher in the spiked digesters and significantly different (p = 0.05) from the control conditions. Specifically, isovaleiic acid concentration was 2 times lower in the control digester compared to the spiked digesters, whereas hydrogen sulfide was 2 times lower in the ENPs spiked digester indicating inhibitory effect on sulfate reducing microorganisms. Based on the ether-linked isoprenoids concentration, the total abundance of methanogens was lA times lower in the ENPs spiked digester than in the control and metal salt spiked digesters. Pyrosequencing indicated 80% decrease in abundance and diversity of methanogens in ENPs spiked digester compared to the control digester. Methanosurcina acetivoruns and Methunosurcina barkeri were identified as nano tolerant as their relative abundance increased by a factor of 6 and 11, respectively, compared to the other digesters. The results further provide compelling evidence on the resilience of Fusobacteria Actinobacteriu and the Trojan horse-like effect of ENPs which offered a competitive advantage to some organisms while reducing microbial abundance and diversity. (C) 2017 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY license (littp://creativecommons.org/licenses/by/4.0/).