Reduced IFN-γ- and enhanced IL-4-producing CD4+ cord blood T cells are associated with a higher risk for atopic dermatitis during the first 2 yr of life
Reduced IFN-γ- and enhanced IL-4-producing CD4+ cord blood T cells are associated with a higher risk for atopic dermatitis during the first 2 yr of life
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DOI：
10.1111/j.1399-3038.2009.00890.x
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发表时间：
2010-02-01
影响因子：
4.4
通讯作者：
Lehmann, Irina
中科院分区：
文献类型：
--
作者：
Herberth, Gunda;Heinrich, Joachim;Lehmann, Irina
这项研究的目的是分析脐带血(CB)T细胞产生的细胞因子变化是否与出生后头两年的变态反应性疾病的发生有关，独立于已知或可疑的过敏危险因素。在一项正在进行的出生队列研究(生活方式-免疫系统-过敏；LISA)中，通过细胞内细胞因子染色测量了PMA/离子霉素刺激的CB细胞的细胞因子产生。对来自莱比锡和慕尼黑的98名儿童的数据进行了分析，这些儿童出生时就有关于细胞因子产生的完整信息，并在最初的两年里出现了过敏反应。统计分析采用回归模型，对性别、出生月份、父母特应性病史、父母教育、接触环境烟草烟雾、孕期母亲吸烟、孕期翻新活动、宠物饲养和研究中心进行了调整。在出生后的头两年，17.3%的儿童患上了医生诊断的特应性皮炎。在CB(第一个四分位数)中产生干扰素-γ的CD4(+)T细胞频率降低的儿童患特应性皮炎的风险更高(调整后的OR为5.16，95%CI：1.04-25.6)。此外，来自莱比锡队列的儿童外周血中产生IL-4的T细胞的高比例与特应性皮炎的风险增加相关(调整后的OR 8.92，95%CI：第90百分位数的1.40-56.93)。CD8(+)细胞因子产生的CB T细胞与特应性皮炎风险增加无关。出生时低剂量的干扰素-γ和高剂量的产生IL-4的T细胞可能会增加随后发生特应性皮炎的风险。
The aim of this study was to analyse whether altered cytokine production by cord blood (CB) T cells is of relevance regarding the development of allergic diseases during the first 2 yr of life independent from known or suspected risk factors for allergy. Within an ongoing birth cohort study (Life style - Immune System - Allergy; LISA) the cytokine production of PMA/ionomycin-stimulated CB cells was measured by intracellular cytokine staining. Data of 98 children from Leipzig and Munich with complete information on cytokine production at birth and allergic outcomes during the first 2 yr were analysed. Statistical analysis was performed using a regression model adjusted for gender, month of birth, parental history of atopy, parental education, exposure to environmental tobacco smoke, maternal smoking during pregnancy, renovation activities during pregnancy, pet ownership and study centre. During the first 2 yr of life, 17.3% of the children developed a physician-diagnosed atopic dermatitis. Children with reduced frequencies of interferon-gamma (IFN-gamma)-producing CD4(+) T cells in the CB (1st quartile) had a higher risk to develop atopic dermatitis (adjusted OR 5.16, 95% CI: 1.04-25.6). Furthermore, a high percentage of interleukin (IL)-4-producing T cells in CB in children from the Leipzig cohort were associated with an increased risk for atopic dermatitis (adjusted OR 8.92, 95% CI: 1.40-56.93 for the 90th percentile). CD8(+) cytokine-producing CB T cells had no relation to increased risk for atopic dermatitis. Low amounts of IFN-gamma and high amounts of IL-4-producing T cells at birth may enhance the risk of subsequent development of atopic dermatitis.