Evidence for the identity of murine gamma interferon and macrophage activating factor.
Evidence for the identity of murine gamma interferon and macrophage activating factor.
复制标题
鼠γ干扰素和巨噬细胞激活因子身份的证据。
DOI：
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发表时间：
1982
期刊：
影响因子：
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通讯作者：
A. Vasil
中科院分区：
文献类型：
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作者：
W. K. Roberts;A. Vasil
使用肿瘤细胞细胞毒性测定对小鼠淋巴因子制剂中的巨噬细胞激活因子（MAF）进行定量。将 MAF 活性与γ干扰素 (IFN-γ) 活性进行比较，并对淋巴因子混合物进行各种蛋白质分级分离程序。即使在 MAF 已纯化至 1 X 10(6) u/mg 蛋白质的比活性后，任何分级步骤后均未观察到 MAF 活性与 IFN 活性之比的显着差异。使用高压液相色谱进行的凝胶渗透显示，MAF 和 IFN 活性在约 55 kD 处有重合峰。在 56 摄氏度下加热或在 pH 2 缓冲液中于 4 摄氏度下孵育后，两种活性均以相似的速率降低。最后，使用不同诱导剂（丝裂原或抗原）或细胞群诱导淋巴因子总是导致 MAF 活性与 IFN 活性的相似比率。这些结果支持 MAF 和 IFN-γ 是相同蛋白质的假设。
Macrophages activating factor (MAF) in mouse lymphokine preparations was quantitated using a tumor cell cytotoxicity assay. MAF activity was compared with gamma interferon (IFN-gamma) activity, and the lymphokine mixture subjected to a variety of protein fractionation procedures. No significant difference in the ratio of MAF activity to IFN activity was observed following any of the fractionation steps, even after MAF had been purified to a specific activity of 1 X 10(6) u/mg protein. Gel permeation using high pressure liquid chromatography showed a coincident peak of MAF and IFN activity at approximately 55 kD. Both activities were reduced at similar rates following heating at 56 degrees C or incubation at 4 degrees C in pH 2 buffer. Finally, induction of lymphokines using different inducers (mitogens or antigens) or cell populations always resulted in similar ratios of MAF activity to IFN activity. These results support the hypothesis that MAF and IFN-gamma are identical proteins.
人伽马（免疫）干扰素的部分纯化和表征。
DOI：
10.1073/pnas.78.3.1601
发表时间：
1981
影响因子：
11.1
作者：
Yip,YK;Pang,RH;Urban,C;Vilcek,J
通讯作者：
Vilcek,J