Anticancer activity of silver(I) cyclohexyldiphenylphosphine complexes toward SNO cancer cells
Anticancer activity of silver(I) cyclohexyldiphenylphosphine complexes toward SNO cancer cells
复制标题
环己基二苯基膦银(I)配合物对SNO癌细胞的抗癌活性
DOI：
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
R. Meijboom
中科院分区：
文献类型：
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作者：
Kariska Potgieter;Zelinda Engelbrecht;G. Naganagowda;M. Cronjé;R. Meijboom
摘要银及其衍生物已经和目前被用作抗微生物和抗菌剂。然而，迄今为止，它们作为抗癌剂的用途相当有限。因此，本研究的重点是合成和表征的三个银（I）配合物，具有采用不同的几何形状的能力，并诱导癌细胞死亡的SNO食管癌细胞。使硫氰酸银以不同比例与环己基二苯基膦反应，得到摩尔比为1：1（络合物1）、1：2（络合物2）和1：3（络合物3）的银（I）：环己基二苯基膦络合物。这些配合物的特征在于使用传统的光谱技术，其中包括1H，13 C，和31 P NMR，FTIR，和微量分析（%C，H，N，S）。此外，还测定了配合物1和2的单晶X-射线结构。此外，所有三种复合物都显示出对恶性SNO细胞的毒性活性，如用alamarBlue®活力测定法测定的，IC 50浓度低于5 μM。包括形态学和流式细胞术分析，以确定癌细胞死亡的可能模式。这些生化分析表明，所有三种治疗诱导细胞凋亡，由于特定的凋亡标志物的存在。
Abstract Silver and its derivatives have been and are currently used as antimicrobial and antibacterial agents. However, their use is rather limited to date as anticancer agents. Thus, this study focuses on the synthesis and characterization of three silver(I) complexes which have the ability to adopt different geometries, and to induce cancer cell death in SNO esophageal cancer cells. Silver thiocyanate was reacted in different ratios with cyclohexyldiphenylphosphine to obtain 1:1 (complex 1), 1:2 (complex 2), and 1:3 (complex 3) molar ratios of silver(I):cyclohexyldiphenylphosphine complexes. These complexes were characterized using conventional spectroscopic techniques, which included 1H, 13C, and 31P NMR, FTIR, and microanalysis (%C, H, N, S). In addition, the single-crystal X-ray structures of complexes 1 and 2 were determined. Moreover, all three complexes displayed toxic activity toward the malignant SNO cells with IC50 concentrations below 5 μM as determined with an alamarBlue® viability assay. Morphological and flow cytometric analyses were included to identify the possible mode of cancer cell death. These biochemical assays revealed that all three treatments induced apoptosis due to the presence of specific apoptotic markers.