A CYCLOPHILIN-RELATED PROTEIN INVOLVED IN THE FUNCTION OF NATURAL-KILLER-CELLS
A CYCLOPHILIN-RELATED PROTEIN INVOLVED IN THE FUNCTION OF NATURAL-KILLER-CELLS
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DOI：
10.1073/pnas.90.2.542
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发表时间：
1993-01-15
影响因子：
11.1
通讯作者：
ORTALDO, JR
中科院分区：
文献类型：
--
作者：
ANDERSON, SK;GALLINGER, S;ORTALDO, JR
自然杀伤细胞是一种非主要组织相容性复合体限制的大颗粒淋巴细胞，可以在没有预先刺激的情况下识别和摧毁肿瘤细胞。人类自然杀伤细胞表面的150 kDa分子被鉴定为假定的肿瘤识别复合体的组成部分。我们在这里报道了从人和小鼠的cDNA文库中分离到编码150 kDa肿瘤识别分子的cDNA。预测的蛋白质的氨基末端含有一个大的疏水区，紧随其后的是一个与亲环素/肽基脯氨酰顺-反异构酶高度同源的结构域。蛋白质的其余部分是极亲水的，包含三个同源的带正电荷的簇。还有三个区域包含大量富含精氨酸和丝氨酸的重复序列。人类和小鼠预测的氨基酸序列的比较显示>；80%的同源性。
Natural killer cells are non-major histocompatibility complex-restricted large granular lymphocytes that can recognize and destroy tumor cells without prior stimulation. A 150-kDa molecule on the surface of human natural killer cells was identified as a component of a putative tumor-recognition complex. We report here the isolation of cDNAs coding for the 150-kDa tumor-recognition molecule from human and mouse cDNA libraries. The amino terminus of the predicted protein contains a large hydrophobic region followed by a domain that is highly homologous to cyclophilin/peptidylprolyl cis-trans isomerase. The remainder of the protein is extremely hydrophilic and contains three homologous positively charged clusters. There are also three regions that contain extensive arginine- and serine-rich repeats. Comparison of the human and mouse predicted amino acid sequences revealed >80% homology.