HLA-DPB1 oligonucleotide typing of a southwest German Caucasian population.
HLA-DPB1 oligonucleotide typing of a southwest German Caucasian population.
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德国西南部白种人群体的 HLA-DPB1 寡核苷酸分型。
DOI：
10.1111/j.1399-0039.1991.tb01897.x
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
S. Goldmann
中科院分区：
文献类型：
--
作者：
T. Eiermann;J. Fakler;C. Müller;M. Ballas;S. Goldmann
应用序列特异性寡核苷酸进行HLA-DP基因分型，可检测DPB 1第二外显子多态性片段中已知的序列变异。由于22个已发表等位基因的等位基因多态性是基于6个可变区序列基序的重组，DPB 1分型取决于许多不同探针的反应模式，而不是用单个等位基因特异性探针分型。通过计算机模拟，我们以前已经表明，定义22个不同等位基因和大多数杂合组合的最小探针集是18。在这里，我们描述了HLA-DPB 1分型结果和等位基因频率在一组200无关的高加索人从西南德国。结果证实了新的HLA-DPB 1分型方法的能力，但我们未能在我们的面板中检测到先前描述的三个等位基因。为了适应与观察到的19个不同的等位基因，序列和杂交条件的17个寡核苷酸探针，这是能够区分所有，除了两个，解决组特异性扩增，190个可能的杂合表型。
HLA-DP genotyping with sequence-specific oligonucleotides can detected known sequence variations in the polymorphic segments of the DPB1 second exon. Since the allelic polymorphism of the 22 published alleles is based on recombination of sequence motifs from six variable regions, DPB1 typing depends on the reactivity pattern of many different probes rather than from typing with single allele-specific probes. By computer simulation, we have previously shown that the minimal set of probes to define the 22 different alleles and most of the heterozygous combinations is 18. Here we describe HLA-DPB1 typing results and allele frequencies in a panel of 200 unrelated Caucasians from Southwest Germany. The result confirmed the power of the new HLA-DPB1 typing method, but we failed to detect three of the previously described alleles in our panel. To accommodate with the observed 19 different alleles, the sequence and hybridization conditions of 17 oligonucleotide probes are given, which are able to differentiate all except two, resolved by group-specific amplification, of the 190 possible heterozygous phenotypes.
原代混合淋巴细胞对 HLA-DP 的反应。
影响因子：
2.7
作者：
Sell,TW;Eckels,DD
通讯作者：
Eckels,DD
通过等位基因特异性或群体特异性扩增对 II 类 HLA 抗原进行 DNA 分型。
影响因子：
2.7
作者：
Fernandez-Vina,M;Moraes,ME;Stastny,P
通讯作者：
Stastny,P