Nectins localize Willin to cell-cell junctions
Nectins localize Willin to cell-cell junctions
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DOI：
10.1111/j.1365-2443.2012.01593.x
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发表时间：
2012-05-01
期刊：
影响因子：
2.1
通讯作者：
Takeichi, Masatoshi
中科院分区：
文献类型：
--
作者：
Ishiuchi, Takashi;Takeichi, Masatoshi
Willin是一种FERM结构域蛋白，与果蝇扩展蛋白（Drosophila Expanded）相关，已知该蛋白是Hippo信号通路的一种组分。我们最近发现，Willin定位在上皮细胞的顶端连接复合体（AJC）与Par3和调节收缩性的圆周肌动球蛋白电缆招募aPKC的AJC。然而，Willin如何与AJC联系在一起仍然没有得到解决。在这里，我们报告说，Willin结合到连接蛋白，Ig家族蛋白，这也本地化在AJC通过其嗜同性或嗜异性的相互作用，这种结合参与交界处招聘的Willin。此外，我们报告说，在AJC的定位的nectin是依赖于他们的结合阿法丁。因此，我们的研究结果表明，nectinafadin相互作用中发挥作用的正确定位的Willin。
Willin is a FERM-domain protein, which is related to the Drosophila Expanded, a protein known to be a component of the Hippo signaling pathway. We recently showed that Willin localizes at the apical junctional complex (AJC) in epithelial cells together with Par3 and regulates the contractility of the circumferential actomyosin cables by recruiting aPKC to the AJC. However, it remains unresolved how Willin becomes associated with the AJC. Here, we report that Willin binds to nectins, Ig-family proteins, which also localize at the AJC via their homophilic or heterophilic interactions, and this binding participates in the junctional recruitment of Willin. In addition, we report that the positioning of nectins at the AJC is dependent on their binding to afadin. Thus, our results suggest that the nectinafadin interaction plays a role in the correct localization of Willin.