Electrical stimulation of denervated rat skeletal muscle ameliorates bone fragility and muscle loss in early-stage disuse musculoskeletal atrophy
Electrical stimulation of denervated rat skeletal muscle ameliorates bone fragility and muscle loss in early-stage disuse musculoskeletal atrophy
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电刺激去神经大鼠骨骼肌可改善早期废用性肌肉骨骼萎缩的骨脆性和肌肉损失
DOI：
10.1007/s00223-017-0250-y
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yamamoto N
中科院分区：
文献类型：
--
作者：
Tamaki H;Yotani K;Ogita F;Hayao K;Nakagawa K;Sugawara K;Kirimoto H;Onishi H;Kasuga N;Yamamoto N
我们测试了日常肌肉电刺激（ES）是否可以改善废用性肌肉骨骼萎缩早期阶段皮质骨强度以及肌肉和骨几何和材料特性的下降。将7周龄雄性F344大鼠随机分为3组：年龄匹配对照组（Cont）、坐骨神经切断组（DN）和DN +直接电刺激组（DN + ES）。DN + ES组大鼠失神经胫骨前肌（TA）接受16 mA、10 Hz的ES，30 min/d，6 d/周。采用微型CT、三点弯曲试验和免疫组织化学来表征胫骨皮质骨的机械、结构和材料特性。与DN组相比，DN + ES组的TA肌肉在肌肉质量和肌纤维横截面积方面显示出显著改善。DN + ES组的胫骨最大载荷和刚度、microCT估计的骨密度和皮质骨组织中的DMP 1免疫反应性也显著高于DN组。这些结果表明，每日ES诱导的肌肉收缩治疗减少了早期废用性肌肉骨骼萎缩中肌肉质量和皮质骨强度的下降，并与材料特性（如皮质骨组织的矿化）的有益影响相关。
We tested whether daily muscle electrical stimulation (ES) can ameliorate the decrease in cortical bone strength as well as muscle and bone geometric and material properties in the early stages of disuse musculoskeletal atrophy. 7-week-old male F344 rats were randomly divided into three groups: age-matched control group (Cont); a sciatic denervation group (DN); and a DN + direct electrical stimulation group (DN + ES). Denervated tibialis anterior (TA) muscle in the DN + ES group received ES with 16 mA at 10 Hz for 30 min/day, 6 days/week. Micro CT, the three-point bending test, and immunohistochemistry were used to characterize cortical bone mechanical, structural, and material properties of tibiae. TA muscle in the DN + ES group showed significant improvement in muscle mass and myofiber cross-sectional area relative to the DN group. Maximal load and stiffness of tibiae, bone mineral density estimated by micro CT, and immunoreactivity of DMP1 in the cortical bone tissue were also significantly greater in the DN + ES group than in the DN group. These results suggest that daily ES-induced muscle contraction treatment reduced the decrease in muscle mass and cortical bone strength in early-stage disuse musculoskeletal atrophy and is associated with a beneficial effect on material properties such as mineralization of cortical bone tissue.