Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription
Maf1 is an essential mediator of diverse signals that repress RNA polymerase III transcription
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DOI：
10.1016/s1097-2765(02)00787-6
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发表时间：
2002-12-01
期刊：
影响因子：
16
通讯作者：
Willis, IM
中科院分区：
文献类型：
--
作者：
Upadhya, R;Lee, JH;Willis, IM
Maf 1是RNA聚合酶（pol）III转录的假定阻遏物，其从酵母到人类都是保守的。在这里，我们表明Maf 1是S. cerevesiae，其感知细胞环境中的变化并抑制pol III转录。响应于雷帕霉素诱导的营养限制、DNA损伤和分泌途径缺陷而激活的信号通路都需要Maf 1以影响pol III转录抑制。此外，Maf 1需要在正常的酵母生长周期中抑制pol III的转录。生物化学研究确定了起始因子TFIIIB作为Maf 1依赖性阻遏的靶点，并揭示了在阻遏条件下TFIIIB-DNA复合物组装的缺陷。
Maf1 is a putative repressor of RNA polymerase (pol) III transcription that is conserved from yeast to humans. Here we show that Maf1 is a common component of multiple signaling pathways in S. cerevesiae that sense changes in the cellular environment and repress pol III transcription. Signaling pathways activated in response to rapamycin-induced nutrient limitation, DNA damage, and secretory pathway defects all require Maf1 in order to affect pol III transcriptional repression. In addition, Maf1 was required for repression of pol III transcription during the normal yeast growth cycle. Biochemical studies identified the initiation factor TFIIIB as a target of Maf1-dependent repression and revealed a defect in TFIIIB-DNA complex assembly under repressing conditions.