Micro/nanofluidic technologies for efficient isolation and detection of circulating tumor cells
Micro/nanofluidic technologies for efficient isolation and detection of circulating tumor cells
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DOI：
10.1016/j.trac.2019.06.009
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发表时间：
2019-08
期刊：
影响因子：
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通讯作者：
Wan Yue;Zheng Tan;Xiu-ping Li;Fei-Fei Liu-Fei;Chen Wang
中科院分区：
文献类型：
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作者：
Wan Yue;Zheng Tan;Xiu-ping Li;Fei-Fei Liu-Fei;Chen Wang
循环肿瘤细胞(CTCs)的灵敏检测对癌症的早期诊断具有重要意义。然而，CTCs的数量在外周血中非常罕见。因此，迫切需要开发新的方法来有效地分离和检测痕量CTCs。在各种方法和分析平台中，微/纳米流体技术在CTCs分离和后续细胞培养以及下游检测中都被证明是一种很有前途的方法。本文综述了基于微/纳米流控技术的CTCs分离检测技术的研究进展。首先介绍了CTCs的特性，然后基于各种原理综述了用于CTCs分离和释放的微/纳米流控技术。然后讨论了CTCs的检测方法，包括荧光、拉曼、电化学检测，以及CTCs的下游表征。最后，我们对CTCS研究的创新微流控平台的未来进行了展望。
Sensitive detection of circulating tumor cells (CTCs) is of great importance for early cancer diagnosis. However, the number of CTCs is very rare in the peripheral blood. Therefore, there is an urgent demand to develop new methods to efficiently isolate and detect trace amount of CTCs. Among various methods and analytical platforms, micro/nanofluidic technologies have been demonstrated as a great promising approach in both CTCs isolation and subsequent cell culture as well as downstream assays. In this review, we summarize the current advances in micro/nanofluidics based technologies for CTCs isolation and detection. The characterizations of CTCs are firstly introduced, and then the micro/nanofluidic technologies for isolation and release of CTCs based on various principles are summarized. After that we discuss the detection methods including fluorescence, Raman, electrochemical detection, and the downstream characterization of CTCs. Finally, we provide a perspective on the future of innovative microfluidic platforms for CTCs study.